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Confining liquid for immune cell p16 staining

A technology of immune cells and blocking solution, applied in the field of liquid-based cytology and immunocytochemistry, which can solve the problems of affecting the p16 staining effect of immune cells, short storage time, and easy deterioration, and achieves enhanced specific binding, long storage time, and prevention of spoilage effect

Inactive Publication Date: 2019-11-26
深圳市森盈生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing protein-based blocking solutions for immune cell p16 staining generally have the defects of easy deterioration, instability, and short storage time, which seriously affect the effect of immune cell p16 staining

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] This embodiment provides a blocking solution for p16 staining of immune cells, which by weight includes 55 parts of PBS buffer, 5 parts of animal non-immune serum, 5 parts of sucrose, 5 parts of Tween-20, 1 part of Proclin300, 20 parts of skimmed milk powder and 100 parts of distilled water.

[0018] In this embodiment, the animal non-immune serum is goat serum with a mass concentration of 5-15%.

[0019] In this embodiment, the mass concentration of the Tween-20 is 0.2%.

[0020] In this embodiment, the concentration of the PBS buffer is 0.01-0.2 mol / L.

[0021] In this embodiment, the mass concentration of Proclin 300 is 0.1%.

[0022] In the above blocking solution for immune cell p16 staining, by adding Proclin300 to the blocking solution, the stability of the blocking solution can be enhanced to prevent the deterioration of nutrients such as animal non-immune serum and skimmed milk powder, thereby making the storage time of the blocking solution Longer. The sucrose can pr...

Embodiment 2

[0025] This embodiment provides a method for preparing the above-mentioned sealing liquid, which includes the following steps:

[0026] S1. First dissolve skimmed milk powder with 1 / 3 of distilled water;

[0027] S2. Use 1 / 3 of distilled water to dissolve sucrose;

[0028] S3. Mix PBS buffer, animal non-immune serum, dissolved sucrose, Tween-20, Proclin300, dissolved skim milk powder and the remaining distilled water, and adjust the pH to 7.2-7.8 to obtain the immunization Blocking solution for cell p16 staining.

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PUM

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Abstract

The invention discloses a confining liquid for immune cell p16 staining, which comprises the following components in parts by weight: 55 parts of a PBS buffer solution, 5 parts of animal non-immune serum, 5 parts of cane sugar, 5 parts of tween-20, 1 part of Proclin300, 20 parts of skim milk powder and 100 parts of distilled water. The stability of the confining liquid can be enhanced by adding Proclin300, and the deterioration of nutritional ingredients such as the animal non-immune serum and the skim milk powder can be prevented, so that the preservation time of the confining liquid can be longer. The cane sugar can play a role in protecting the animal non-immune serum and the skim milk powder, so that the animal non-immune serum and the skim milk powder are more stable. The cane sugar can also play a role in resisting oxidation when the confining liquid is in contact with air, so that the animal non-immune serum and the skim milk powder are protected. In addition, Tween-20 is addedto be combined with the animal non-immune serum and the skim milk powder for use, so that the effect of cleaning non-specific binding or loosely bound protein can be achieved during confining, and thespecific binding is enhanced.

Description

Technical field [0001] The invention relates to the field of liquid-based cytology and immunocytochemistry, in particular to a blocking solution for p16 staining of immune cells. Background technique [0002] Cervical cancer is one of the most common malignant tumors in gynecology. Its incidence is second only to breast cancer and shows a younger trend, which seriously endangers women's health. However, cervical cancer is the only clear cause and is expected to be the first tumor to be conquered by humans. Therefore, prevention and early screening are of great significance to reduce the incidence and mortality of cervical cancer. [0003] At present, the main methods of cervical cancer diagnosis include: cervical exfoliation cytology, human papillomavirus (HPV) detection, colposcopy, pathological biopsy, etc. Cytology is mainly used by pathologists to stain the cervical exfoliated cells and then observe and diagnose under a microscope. This method is relatively simple to operate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 黄子权
Owner 深圳市森盈生物科技有限公司