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A mouse model of Alzheimer's disease and its evaluation method

A technology of mouse model and evaluation method, which is applied in the fields of pharmaceutical formulation, compound screening/testing, animal husbandry, etc., and can solve problems such as expensive, time-consuming, non-homozygous, etc.

Active Publication Date: 2021-12-17
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the most widely used is the transgenic mouse model, but due to its expensive, time-consuming, non-homozygous and other defects, it brings certain research limitations

Method used

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  • A mouse model of Alzheimer's disease and its evaluation method
  • A mouse model of Alzheimer's disease and its evaluation method
  • A mouse model of Alzheimer's disease and its evaluation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1, the present invention is in vitro Zn 2+ Combined with tau-R3 and injected into the left cortex and right hippocampus of six-month-old C57 mice. After 45 days, a series of mouse behavior and biochemical indicators were detected.

[0026] 1.1. Open field test: the number of grids crossed, the number of times of standing and the number of defecation of mice in each group.

[0027] 1.2. Elevated plus maze experiment: the time when each group of mice entered the open (closed) arm for the first time, the total distance of entering the open (closed) arm, the time of staying in the open (closed) arm and the number of times of entering the open (closed) arm .

[0028] 1.3. New object recognition experiment: the total exploration time of each group of mice in the familiarization period and the discrimination ratio of old and new objects in the test period.

[0029] 1.4. Scenario fear test: the total freezing time of each group of mice in the association test, change associa...

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Abstract

A mouse model of Alzheimer's disease first introduced by Zn 2+ Combines with tau-R3 to form a tau oligomer (Zn 2+ ‑R3), and the formed oligomers were injected into the left cerebral cortex and right cerebral hippocampus of six-month-old C57 mice. After 45 days, a series of mouse behavior and biochemical indicators were detected. The Alzheimer's disease mouse model of the present invention has simple preparation method, low price and short duration, and can be used as an ideal tau pathological model, providing a certain experimental basis for mechanism exploration and drug research of AD and other tau pathological diseases .

Description

technical field [0001] The present invention relates to a mouse model of Alzheimer's disease. [0002] The present invention also relates to a method for evaluating the above mouse model of Alzheimer's disease. Background technique [0003] Tau protein is a microtubule-associated protein whose main function is to combine with tubulin to assemble into microtubules, maintain the stability of microtubules and prevent the dissociation of tubulin. The pathology of neurofibrillary tangles (NFT) formed by the aggregation of hyperphosphorylated tau protein is closely related to a variety of central nervous system diseases, such as Alzheimer's disease (Alzheimer's disease, AD), Pick's disease, progressive supranuclear palsy , corticobasal degeneration, traumatic encephalopathy, epilepsy, frontotemporal dementia, etc., the most common of which is AD. In these diseases, the distribution and amount of tau pathology are closely related to the severity and phenotype of cognitive impairm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/027A61K49/0008A01K2207/10A01K2227/105A01K2267/0312
Inventor 都秀波李雪霞倪嘉缵
Owner SHENZHEN UNIV
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