Application of tanshinol as effective component of boar semen normal-temperature antibacterial preserving fluid

A technology of active ingredients and preservation solution, applied in the application of Danshensu as a diluent component for boar semen preservation at room temperature, and the field of boar semen preservation diluent at room temperature, can solve problems such as insufficient understanding of antibiotics, excessive antibiotic content, and excessive antibiotics, etc. To achieve the effect of improving boar sperm motility

Inactive Publication Date: 2019-12-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the lack of understanding of antibiotics, humans use antibiotics to randomly add in the process of livestock breeding, resulting in excessive levels of antibiotics in livestock, and eventually human consumption leads to excessive levels of antibiotics in humans

Method used

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  • Application of tanshinol as effective component of boar semen normal-temperature antibacterial preserving fluid
  • Application of tanshinol as effective component of boar semen normal-temperature antibacterial preserving fluid
  • Application of tanshinol as effective component of boar semen normal-temperature antibacterial preserving fluid

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Embodiment

[0016] According to the technical scheme of the present invention, this embodiment provides seven groups of diluent formulations using Danshensu for boar semen stored at room temperature, and the concentrations of Danshensu added to the base liquid are 0 μM, 1 μM, 5 μM, 10 μM, 25 μM, 50 μM, 100 μM, as shown in Table 1. Weigh other components in each 1L of dilution according to Table 2.

[0017] Table 1 Danshensu addition concentration scheme

[0018]

[0019] Table 2 Basic Diluent Formula

[0020]

[0021] The normal temperature preservation diluent of boar semen in the above-mentioned embodiment is used for the method for the normal temperature preservation of boar semen, implements according to the following steps:

[0022] 1. Preparation of basic diluent: Accurately weigh D-glucose, citric acid, trisodium citrate, sodium bicarbonate, potassium chloride, EDTA, and Tris in a sterilized beaker according to the composition of the medium-effect dilution powder, and take ...

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Abstract

The invention relates to an application of tanshinol as an effective component of a boar semen normal-temperature antibacterial preserving fluid. According to the invention, tanshinol is found to be capable of simultaneously exerting antioxidant and antibacterial effects in a normal-temperature preservation process of boar semen, quality of the semen can be improved, and the effective preservationtime can be prolonged to 9 days.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a diluent for storing boar semen at room temperature, in particular to the application of danshensu as a component of the diluent for storing boar semen at room temperature. Background technique [0002] With the promotion and application of pig artificial insemination technology in intensive pig farms, more and more attention has been paid to the preservation technology of semen, and it has increasingly shown that it can reduce the amount of boar breeding and feeding costs, accelerate the improvement of pig breeds, reduce the Advantages in many aspects such as disease transmission. Compared with frozen semen, semen preserved in liquid state at room temperature has the advantages of high reproductive performance of sows (high conception rate, farrowing rate and litter size), low cost and simple operation procedures after artificial insemination; considering the effect of semen preserva...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0215A01N1/0226
Inventor 胡建宏陈美杰张利坤贺巾津冯天雨孙超任发席华明李宇杨公社
Owner NORTHWEST A & F UNIV
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