Application of tanshinol as effective component of boar semen normal-temperature antibacterial preserving fluid
A technology of active ingredients and preservation solution, applied in the application of Danshensu as a diluent component for boar semen preservation at room temperature, and the field of boar semen preservation diluent at room temperature, can solve problems such as insufficient understanding of antibiotics, excessive antibiotic content, and excessive antibiotics, etc. To achieve the effect of improving boar sperm motility
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[0016] According to the technical scheme of the present invention, this embodiment provides seven groups of diluent formulations using Danshensu for boar semen stored at room temperature, and the concentrations of Danshensu added to the base liquid are 0 μM, 1 μM, 5 μM, 10 μM, 25 μM, 50 μM, 100 μM, as shown in Table 1. Weigh other components in each 1L of dilution according to Table 2.
[0017] Table 1 Danshensu addition concentration scheme
[0018]
[0019] Table 2 Basic Diluent Formula
[0020]
[0021] The normal temperature preservation diluent of boar semen in the above-mentioned embodiment is used for the method for the normal temperature preservation of boar semen, implements according to the following steps:
[0022] 1. Preparation of basic diluent: Accurately weigh D-glucose, citric acid, trisodium citrate, sodium bicarbonate, potassium chloride, EDTA, and Tris in a sterilized beaker according to the composition of the medium-effect dilution powder, and take ...
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