Method for efficiently using ricefield eel sperms

A technology for sperm and eel, applied in the field of fish farming, can solve the problems of wasting sperm, reducing fertilization rate, production loss, etc., and achieve the effects of cost saving, improving fertilization rate and efficient utilization

Active Publication Date: 2019-12-20
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

Therefore, in large-scale production, if the eel sperm quality is not detected and calculated, it will not only waste sperm, but also reduce the fertilization rate, resulting in production loss

Method used

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Embodiment Construction

[0016] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0017] Concrete implementation method of the present invention is:

[0018] 1. Configure sperm preservation solution; the preparation method of sperm preservation solution is to mix 1 gram of anhydrous calcium chloride, 9 grams of sodium chloride, 0.3 grams of potassium chloride, 1.5 grams of glucose, and 0.4 grams of sodium bicarbonate, then add pure water Dilute to 1 liter and store frozen;

[0019] 2. Kill the male field eel and take out the testis;

[0020] 3. Place 5-7 ml of pre-cooled sperm preservation solution in a 10 ml centrifuge tube;

[0021] 4. Hold the testis with tweezers, squeeze all the semen in the testis into a centrifuge tube, fill the centrifuge tube with pre-cooled sperm preservation solution to 10 ml, and obtain a sperm mixture;

[002...

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Abstract

The invention relates to a method for efficiently using ricefield eel sperms. The method comprises the following steps: 1, preparing a sperm preservation solution and carrying out freezing preservation; 2, taking out the pre-frozen sperm preservation solution, and adding semen of male ricefield eel into the pre-frozen sperm preservation solution to obtain a sperm mixed solution; 3, checking spermmotility and density by using a microscope, and calculating the number of configurable ricefield eel eggs of the sperm mixed solution; and 4, diluting the sperm mixed solution according to the configurable number of the finless eel eggs and carrying out preservation. The invention designs a method for efficiently utilizing ricefield eel sperms, cost is saved, and the fertilization rate of ricefield eel is increased. Through use of the ricefield eel sperms by the method, the sperms can be preserved for 72 hours at a temperature of 4 DEG C and can be available at any time, and the fertilizationrate reaches 95% or above.

Description

technical field [0001] The invention relates to the field of fish farming, in particular to a method for efficiently using eel sperm. Background technique [0002] Field eel is a hermaphroditic sex-reversed fish. In the early stage of the development of field eel, females can lay eggs. After laying eggs, the ovary gradually turns into a testis to produce sperm. There is asynchronous development between female and male eels, and the testes of eels are smaller and produce less sperm. Therefore, in large-scale production, if the eel sperm quality is not detected and calculated, it will not only waste sperm, but also reduce the fertilization rate, resulting in production loss. How to effectively use eel sperm to improve the fertilization rate is not only a scientific problem, but also an economic problem. Therefore, it is very necessary and valuable to invent a method for efficiently utilizing eel sperm to improve the fertilization rate. Contents of the invention [0003] T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 李忠
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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