A kind of engineering bacteria and method for producing Danshensu

A technology of danshensu and genetically engineered bacteria, applied in the field of bioengineering, can solve the problems of high cost of 3,4-dihydroxyphenylpyruvate intermediate, increase the complexity of catalytic reaction, increase the cost of industrial application, etc., and achieve good industrial application Foreground, simple process, and easy-to-obtain raw materials
CN110592038BActive Publication Date: 2021-03-26卓虹超源生物科技(郑州)有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
卓虹超源生物科技(郑州)有限公司
Publication Date
2021-03-26

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Abstract

The invention discloses an engineering bacterium and a method for efficiently producing Danshensu, and belongs to the technical field of bioengineering. The invention constructs a novel double-enzymeco-expression genetically engineered bacterium which can be applied to the production of optically pure Danshensu. The (D / L)-[alpha]-hydroxy carboxylic acid dehydrogenase selected by the invention hasthe characteristics of poor substrate specificity and high optical specificity, and optically pure R-Danshensu and S-Danshensu can be produced. By use of the method for producing Danshensu through the transformation of recombinant bacteria, a process is simple, raw materials can be easily obtained, impurities are few, and the method has an important industrial application value.
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Description

technical field

[0001] The invention relates to an engineering bacterium and a method for producing danshensu, belonging to the technical field of bioengineering. Background technique

[0002] Danshensu extracted from Danshen, scientific name R-(+)-3-(3,4-dihydroxyphenyl)-2-hydroxypropionic acid, D-(+)-β-(3,4-dihydroxyphenyl ) lactic acid, English name: Danshensu, D-DSS, R-DSS, (R)-(+)-3-(3,4-Dihydroxyphenyl)-lactic acid, (R)-(+)-3-(3 ,4-Dihydroxyphenyl)-2-hydroxypropanoic acid is a dextrophenolic acid compound. Natural levodanshensu does not currently exist.

[0003] Danshensu is an important active ingredient in the water extract of Danshen, and its structure was obtained and identified in China in 1980 from the water extract of Danshen (Study on the water-soluble active ingredients of Danshen, Ⅱ.D(+)β(3,4-di The structure of hydroxyphenyl) lactic acid, Shanghai First Medical College Journal, 1980, 05 (7), 384-385), various studies have shown that Danshensu has importan...

Claims

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