Human intestinal flora quantitative detection method and application thereof
A quantitative detection method, the technology of intestinal flora, applied in the field of microbial detection, can solve the problems of intestinal health management that cannot effectively evaluate human intestinal health, time-consuming, and difficult to accurately judge, so as to contribute to intestinal health management effect
Pending Publication Date: 2019-12-20
中诺(杭州)基因科技有限责任公司
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Problems solved by technology
The current monitoring methods are mainly based on the qualitative analysis of direct smear and the quantitative inspection of bacterial culture, which is time-consuming and difficult to judge accurately
Some research institutions choose to use 16S, fingerprints, NGS, methane and hydrogen exhalation methods for early diagnosis of normal flora and flora imbalance in order to find reliable biomarkers. The operation is cumbersome and cannot effectively evaluate human intestinal health status and intestinal health management
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Embodiment Construction
[0023] The present invention is a method for quantitative detection of human intestinal flora, including the following steps:
[0024] S01. Stool sample collection, storage and labeling, collect feces to be tested from people with diarrhea, take 1g of the end of feces as a feces sample, put the fecal sample into the collection tube and add the fecal sample and the fecal preservation solution according to W / V as 3 :1 ratio of fecal preservation solution is shaken, and the user information of the fecal sample is marked for inspection;
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The invention discloses a human intestinal flora quantitative detection method and an application thereof. The human intestinal flora quantitative detection method comprises the following steps of S01, collecting excrement and urine samples, and performing storing and labeling; S02, treating the excrement and urine samples and performing detecting: taking excrement and urine sample supernatant, adding internal reference bacterial strains Sa, extracting nucleic acid with a magnetic bead method excrement and urine DNA extraction reagent kit, setting a negative control group, an internal reference bacterial strain group and a plurality of standard groups, determining the concentration of each group, and then performing fluorescent PCR detection; and S03, analyzing detection results: countingthe content of bacteria to be detected, determining the flora scope difference of the excrement and urine samples and the healthy crowds, so as to assess the intestine health condition of target population. The detection method is applied to detect the content of flora in excrement and urine of the human bodies, and the content is compared with the reference range of the flora of the healthy crowds, so that the health condition of the intestine microecology of the human bodies is assessed. The method has the advantages of being capable of effectively detecting the quantity of the intestinal floras to further assess the health condition of the intestines of the human bodies, and favorable for health management of the intestines.
Description
【Technical Field】 [0001] The invention relates to the technical field of microbial detection, in particular to the technical field of a quantitative detection method and application of human intestinal flora. 【Background technique】 [0002] There are a large number of symbiotic microorganisms in the human intestinal tract, and they affect human health through various ways. In particular, various metabolites play an important role in the promotion of human health and the occurrence and development of diseases. Intestinal microecology studies have found that diabetes, irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), obesity, depression and other diseases are all related to intestinal microecology. The latest research has found that Alzheimer’s disease may be related to Intestinal microbes are related, and the intestinal microecological balance is becoming an important indicator of human health. Maintaining the dynamic balance of the intestinal microecology helps t...
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IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04
CPCC12Q1/689C12Q1/686C12Q2563/107C12Q2545/114Y02A50/30
Inventor 李强朱罗罗徐威张辉林清源
Owner 中诺(杭州)基因科技有限责任公司