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Method for judging activity of activated sludge or biological membrane by trypan blue

A technology of activated sludge and trypan blue, applied in chemical instruments and methods, preparation of test samples, biological water/sewage treatment, etc., can solve the problems of obvious time-consuming and obvious hysteresis.

Inactive Publication Date: 2019-12-20
上海济旦水科技有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] In addition, although microbiological methods such as plate counting and separation culture can reflect the microbial indicators and activities of activated sludge or biofilm, such methods are time-consuming and lagging. Except for the determination of Escherichia coli or coliform bacteria in effluent, such means are rarely used
[0006] At present, there is no convenient and time-effective method to supplement or replace the indicators for judging the activity of activated sludge or biofilm, so as to improve the accuracy and convenience of judging the activity of activated sludge or biofilm

Method used

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  • Method for judging activity of activated sludge or biological membrane by trypan blue
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  • Method for judging activity of activated sludge or biological membrane by trypan blue

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Embodiment 1

[0034] The present invention is applied to the detailed operation flow of activated sludge activity judgment

[0035] Sampling: Use a deep water sampler to take 1L of activated sludge-raw water mixture in the biological pool of the sewage treatment plant, and transfer it to a container with a suitable volume (not less than 1.2L) and shake it well;

[0036] Staining: Immediately take 1ml of the above-mentioned fully shaken activated sludge-raw water mixture, add it into a test tube with a suitable volume (3-5ml), and add 0.1ml of 0.4% trypan blue solution, shake it well and let it stand for 3min;

[0037] Microscopic examination: Shake the test tube again, take an appropriate amount of the above-mentioned stained activated sludge-raw water mixture, drop it on a clean slide, cover it with a cover glass, and place it under a microscope with a 10x eyepiece plus 4 Objective lens (magnification is 40 times) for observation, or use 10 times eyepiece plus 10 times objective lens (magn...

Embodiment 2

[0042]Using the method provided by the invention to judge the activity of activated sludge during the fault debugging of a sewage treatment plant in Shanghai

[0043] In March 2019, a sewage treatment plant in Shanghai had an operational failure, and the quality of the effluent could not meet the standard. After the discussion of the sewage treatment plant and its upgrading and transformation project department, it was decided to carry out debugging including but not limited to adding microbial agents, increasing internal backflow, and increasing aeration. During this period, the method provided by the present invention was adopted to check its sludge activity.

[0044] The time period for using the present invention to inspect the activated sludge of the sewage treatment plant is from April 5, 2019 to April 10, 2019, when the effluent reaches the standard. The phenomenon observed during the period is: the trypan blue staining rate of the activated sludge samples gradually de...

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Abstract

The invention relates to a method for judging the activity of activated sludge or a biological membrane by trypan blue, and belongs to the technical field of sewage treatment. The method comprises thefollowing steps: dyeing the activated sludge or a biological membrane by using a trypan blue solution, inspecting by using an optical microscope, counting the trypan blue dyeing rate, prompting thatthe activity of the tested activated sludge or the biological membrane is poorer if the dyeing rate is higher, and otherwise, prompting that the activity of the tested activated sludge or the biological membrane is higher. The method is convenient, has timeliness and is high in accuracy.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a method for judging the activity of activated sludge or biofilm with trypan blue. Background technique [0002] Sewage treatment plants that use various biological treatment methods often need to measure some indicators of activated sludge or biofilm by various physical and chemical means in the process of operation and commissioning in order to judge their activity. These methods and indicators are currently used more There are many: determination of sludge concentration (MLSS), volatile sludge concentration (MLVSS), sludge sedimentation ratio (SV), sludge volume index (SVI) and other indicators, and microscopic observation of biological equivalence. [0003] However, the above indicators such as sludge concentration (MLSS), volatile sludge concentration (MLVSS), sludge sedimentation ratio (SV), and sludge volume index (SVI) are superficial phenomena. It can always re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/29G01N1/30C02F3/00
CPCC02F3/00G01N1/30G01N21/29
Inventor 刘宫介张显忠冯雷雨
Owner 上海济旦水科技有限公司
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