Application of dendrobium officinale and extract thereof to improvement of diabetes and metabolic syndrome in combination with probiotics

A technology for metabolic syndrome and Dendrobium officinale, applied in the field of medicine

Pending Publication Date: 2019-12-24
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dendrobium officinale and probiotics have complementary medicinal effects, but so far, no reports have found that the

Method used

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  • Application of dendrobium officinale and extract thereof to improvement of diabetes and metabolic syndrome in combination with probiotics
  • Application of dendrobium officinale and extract thereof to improvement of diabetes and metabolic syndrome in combination with probiotics
  • Application of dendrobium officinale and extract thereof to improvement of diabetes and metabolic syndrome in combination with probiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 In this experiment, Bifidobacterium and Streptococcus thermophilus were taken as examples. Dendrobium officinale and its extracts promote the growth of probiotics and inhibit the growth of harmful bacteria

[0023] Resuscitate Bifidobacterium, Streptococcus thermophilus, Escherichia coli and Staphylococcus aureus, use lactic acid bacteria medium for Bifidobacterium, M17 medium for Streptococcus thermophilus, LB medium for Escherichia coli, and beef extract peptone for Staphylococcus aureus culture medium. After overnight recovery at 37°C, they were added to the medium at a ratio of 1:1000, and different concentrations (0, 0.01, 0.05, 0.1, 0.5, 1 mg / mL) of Dendrobium officinale extract were given, and 6 parallel experiments were set up for each concentration. Wells, after growing at 37°C for 8h, adopt OD 600 Detect the growth of Bifidobacterium, Streptococcus thermophilus, Escherichia coli and Staphylococcus aureus.

[0024] Table 1 The effect of Dendrobium o...

Embodiment 2

[0028] Example 2 Dendrobium officinale and its extract combined with probiotics to control weight gain

[0029] Select 5-week-old male C57 mice (Qinglongshan Animal Experiment Center, Nanjing, Jiangsu), weighing 20-24 g, male, and divide them into normal group, control group (fed with high-fat feed), administration group 1 (extracted from Dendrobium officinale) medicine), administration group 2 (probiotics) and administration group 3 (combined action of Dendrobium officinale and probiotics), 10 rats in each group, half male and half male. The normal group was given a normal diet, and the other four groups were fed with high-fat feed. While feeding the high-fat feed, different drugs were given orally, and the body weight of mice in each group was recorded every 2 weeks for a total of 12 weeks. The body weight growth of the mice in the drug group.

[0030] Table 2 Effect of Dendrobium officinale extract combined with probiotics on weight gain of mice

[0031]

[0032] *Comp...

Embodiment 3

[0034] Example 3 Dendrobium officinale and its extracts combined with probiotics reduce blood sugar in diabetic mice induced by high-fat diet

[0035] Oral glucose tolerance test (OGTT) in high-fat diet-induced diabetic mice: 5-week-old C57 mice (Qinglongshan Animal Experiment Center, Nanjing, Jiangsu), weighing 20-24 g, male, were used to induce obesity in mice with high-fat diet . The modeled mice were randomly divided into 4 groups, which were control group, probiotic group (100mg / kg), dendrobium group (100mg / kg), dendrobium extract combined probiotic group (100mg / kg+100mg / kg), Eight rats in each group were administered orally every day for 12 consecutive weeks. At the end of the experiment, the mice were fasted without food and water for 12 hours before the experiment, administered glucose (2 g / kg) by gavage, and blood was collected from the tail to measure the blood sugar level at 0, 15, 30, 60, and 120 minutes after the administration.

[0036] Table 3 Effect of Dendro...

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Abstract

The invention relates to the technical field of biology, in particular to dendrobium officinale and a dendrobium officinale extract combined probiotics for regulating blood sugar and blood fat, and improving diabetes and metabolic syndrome and alleviating occurrence of related complications so as to maintain metabolic stability of organisms. The dendrobium officinale has the effects of regulatingand controlling blood sugar, enhancing body immunity and the like; the probiotics is an active microorganism beneficial to a host, can regulate intestinal flora balance, and can generate an exact health effect so as to improve the micro-ecological balance of the host and play a beneficial role. According to the invention, the dendrobium officinale exerts the effects of reducing blood sugar and blood fat, prebiotics can promote the growth of probiotics and regulate glucose uptake and improve the gastrointestinal irritation caused by taking the probiotics alone, so that the probiotics and dendrobium officinale can better play the role in reducing blood sugar, complications are reduced, and a pharmaceutical composition for preventing or treating diabetes mellitus and metabolic syndromes can be prepared by the probiotics and dendrobium officinale. Therefore, the product provides a new application for improving diabetes and metabolic syndrome related diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a new application of dendrobium officinale combined with probiotics to improve complications related to diabetes and metabolic syndrome. Background technique [0002] Diabetes is a disease of energy metabolism, which is mainly divided into type 1 diabetes (insulin-dependent diabetes) and type 2 diabetes (non-insulin-dependent diabetes). At present, there are about 366 million diabetic patients in the world, accounting for 6.4% of the world's population, and type 2 diabetic patients account for about 90-95% of the total number of diabetic patients. The pathogenesis of type 2 diabetes is very complex. In addition to changes in the human genome, eating habits or reduced physical activity leading to diabetes, changes in the structure of intestinal flora are related to the occurrence and development of diabetes. As an environmental factor, intestinal flora can significan...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61K45/06A61P3/10A61P3/00A61P3/06A61P1/16A61P3/04A61P7/00A61P19/06A61P1/08A61P27/02A61P13/12A61P25/00A61K35/745A61K35/747A61K35/744
CPCA61K35/744A61K35/745A61K35/747A61K36/8984A61K45/06A61P1/08A61P1/16A61P3/00A61P3/04A61P3/06A61P3/10A61P7/00A61P13/12A61P19/06A61P25/00A61P27/02A61K2300/00
Inventor 徐郁蕊张瑜于倩倩刘晓平宁兴海
Owner NANJING UNIV
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