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In-situ microorganism degradation preparation for soil contaminated by sulfonamide antibiotics, preparation method and application thereof

A technology of polluted soil and antibiotics, applied in the restoration of polluted soil, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as blanks

Active Publication Date: 2019-12-27
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The preparation of in-situ microbial digestion preparations for soil contaminated by sulfonamide antibiotics is even blank

Method used

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  • In-situ microorganism degradation preparation for soil contaminated by sulfonamide antibiotics, preparation method and application thereof
  • In-situ microorganism degradation preparation for soil contaminated by sulfonamide antibiotics, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0059] (1) Preparation of sulfonamide antibiotics degrading bacterial population

[0060] Bacillus subtilis subsp.subtilis CICC23830, Pseudomonas sp JR-3, Aeromonas caviae-HA-J1 (already in the literature "He Yanyan. Hong Screening of sulfamethoxine (SMMX) degrading bacteria in forest sediments and research on its degradation performance. "Guangdong Ocean University", 2015 "published), Aeromonas (Aeromonas sp.) CICC 23565, Rhodococcus ( Rhodococcus ruber) CICC 23622, Rhodococcus equi ATCC 13557, Stenotrophomonas acidaminiphila-BX3 (already in the literature "Zhang Qingming. Isolation, identification and analysis of fomesafen-degrading bacteria BX3 Research on degradation characteristics. Journal of North China Agricultural Science, 2013 (3) "published), Myrothecium verrucaria CICC 2625, Albifimbria verrucaria CGMCC 3.1954, Lysinibacillus sp.-ZB-1 (Already in the literature "B Liang, P Lu, H Li,, et al.Biodegradation offomesafen by strain Lysinibacillus sp.ZB-1isolated from so...

Embodiment 2

[0068] (1) Preparation of sulfonamide antibiotics degrading bacterial population

[0069] Bacillus subtilis subsp.subtilis CICC23830, Aeromonas sp. CICC 23565, Rhodococcus ruber CICC23622, Rhodococus equi ATCC 13557, verrucous varnish Albifimbria verrucaria CGMCC 3.1954 and other five degrading bacteria were cultured in LB to the logarithmic phase, and the culture was carried out at 30°C and 130r / min shaking conditions to obtain the culture solution of each bacteria. Then, carry out domestication: A, get respectively 200 μ L each bacterium culture solution in 250mL Erlenmeyer flask, wherein high concentration sulfathiazole (440mg / L) soil suspension has been housed (soil suspension is that soil water is mixed by mass ratio 1:5) Ratio, obtained by sterilization) 100mL, carry out wild domestication for 10 days (the domestication condition is 28 ℃, under the condition of 140r / min shaking); B, take 200 μ L of wild domestication solution and repeat step A 6 times, then place it in L...

Embodiment 3

[0077] (1) Preparation of sulfa antibiotic-degrading bacterial population

[0078] Bacillus subtilis subsp.subtilis CICC23830, Pseudomonas sp. JR-3, Aeromonas sp. CICC 23565, Rhodococus equi ATCC 13557 , Stenotrophomonasacidaminiphila-BX3, Myrothecium verrucaria CICC 2625, Lysinibacillus sp.-ZB-1 and other single degrading bacteria were cultured in LB liquid medium until In the logarithmic phase, the culture was carried out at 28°C and 140r / min shaking conditions to obtain the culture solution of each bacteria. Then, carry out domestication: A, get 200 μ L each bacterium culture solution in 250mL Erlenmeyer flask, wherein the soil suspension 100mL of the soil-water ratio 1:5 (mass ratio) of sterilizing has been housed, contains high concentration sulfamethazine ( 460mg / L), carry out wild domestication for 8 days (the domestication condition is 29 ℃, carry out under the shaking condition of 150r / min); Shake culture for 60 hours to prepare a single degrading bacteria seed stoc...

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Abstract

The present invention discloses an in-situ microorganism degradation preparation for soil contaminated by sulfonamide antibiotics, a preparation method and an application thereof. A sulfonamide antibiotic-degrading microorganism solution, an energy supply bacterial solution, a magnetic medium bacterial solution, a surfactant and a fermentation culture medium are mixed to obtain a mixed solution; and fermentation is performed to obtain an in-situ microorganism degradation preparation for soil contaminated by sulfonamide antibiotics. The sulfonamide antibiotic-degrading microorganisms can significantly improve degradation efficiency of microorganisms through accompanying and coupled fermentation of the energy supply bacteria and improve colonization and proliferation ability of the degrading strains in in-situ soil environment; the surfactant and magnetic medium bacteria can help the degradation preparation relieve water tension and antibiotic polarity after the degradation preparationis applied into soil, enhance affinity of sulfonamide antibiotic residues and degrading microorganisms, and accelerate degradation of the sulfonamide antibiotic residues; and the sulfonamide antibiotic-degrading microorganisms can produce laccase and have superior performances of wide oxidation substrates, low energy consumption, high efficiency, environmental friendliness, etc. on the sulfonamideantibiotic degradation.

Description

technical field [0001] The invention belongs to the field of soil organic pollutant treatment in environmental protection, and particularly relates to an in-situ microbial digestion preparation for sulfonamide antibiotic-contaminated soil, a preparation method and application thereof. Background technique [0002] Sulfonamide antibiotics are a class of artificially synthesized antibacterial drugs that were used relatively early. They all have a p-aminobenzenesulfonamide structure and are competitive inhibitors of p-aminobenzoic acid, which can affect the synthesis and metabolism of tetrahydrofolate and inhibit The growth and reproduction process of bacteria. Sulfonamide antibiotics have a wide antibacterial spectrum, stable performance, convenient use, high efficiency, low price, and easy long-term storage. They are widely used in human medicine and veterinary medicine among several types of antibiotics. The main sulfonamide antibiotics are sulfadiazine (SD), sulfapyridine ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/125C12R1/38C12R1/01C12R1/15C12R1/13C12R1/39
CPCB09C1/10B09C2101/00C12N1/20
Inventor 解开治徐培智李文英顾文杰卢钰升李夏孙丽丽蒋瑞萍卢廷超
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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