A culture method for improving the survival rate of mammalian embryos in vitro

Abstract

The invention discloses a culture method for improving the survival rate of mammalian embryos in vitro, which comprises the following steps: (1) taking sheep oocytes from ewes and putting them into a mature culture solution, culturing them in an incubator for 20-24 hours, Obtain mature sheep oocytes; (2) Put the mature sheep oocytes and sperm into the fertilization medium, culture them in the incubator for 18‑24 hours, and fertilize them to obtain early sheep embryos; (3) Put the The fertilized sheep embryos were washed repeatedly with TCM199 culture medium, then put into sheep embryo in vitro culture medium, and placed in an incubator for in vitro culture of embryos. The temperature in the incubator was maintained at 38-39°C, and the gas concentration was maintained at 5% CO2; wherein, the above-mentioned incubator includes a cultivation chamber, a chamber door, and a gas concentration adjustment chamber connected with the cultivation chamber; there are multiple cultivation chambers in the cultivation chamber, and a culture medium is respectively placed in the cultivation chambers racks and partitions. The stability of the environment in the incubator of the present invention ensures the survival rate of embryos.

Description

technical field [0001] The invention belongs to the technical field of in vitro culture of sheep embryos, in particular to a culture method for improving the in vitro survival rate of mammalian embryos. Background technique [0002] In vitro culture refers to placing embryos in an incubator, which maintains a constant temperature and a constant concentration of carbon dioxide, simulating the physiological environment of the uterus, and the embryos in the culture medium interact and exchange substances with it, thereby achieving embryo growth. In the existing culture method, after the embryos are placed in the incubator, the operator needs to observe or operate the embryos frequently. At this time, the door of the incubator needs to be opened, and during the operation, the environment in the box will change. The gas exchange with the outside air causes the environment inside the box to be affected and changes. It is difficult to maintain a stable temperature and atmosphere in...

AI Technical Summary

Problems solved by technology

In the existing culture method, after the embryos are placed in the incubator, the operator needs to observe or operate the embryos frequently. At this time, the door of the incubator needs to be opened, and during the operation, the environment in the box will change. The gas exchange with the outside air makes the environment in the box affected and changes, it is difficult to ensure that the incubator is always maintained in a stable temperature and atmosphere environment, the embryos are prone to damage or death, and the embryo survival rate is low

Images