Method for extracting total RNA from root system of magnolia cylindrical
An extraction method and root technology, which are applied in the field of extraction of total RNA from the roots of Magnolia japonica, can solve problems such as inability to meet, and achieve the effects of improving extraction rate and purity, high extraction rate, and simple and easy extraction method.
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Embodiment 1
[0072] A method for extracting total RNA from the root system of Magnolia huangshan, comprising the following steps:
[0073] 1) Dissolve 2.4g Tris in 1 / 1000 DEPC water of 100ml, add hydrochloric acid to adjust to pH=8, obtain Tris-HCl buffer solution; Dissolve 1.86gEDTA-Na 2 2H 2 O was dissolved in 100ml of DEPC water, and hydrochloric acid was added to adjust the pH to 8 to obtain EDTA-Na 2 Solution, mix the two solutions prepared above, add 4g CTAB, 4g PVP and 23.4gNaCl, mix well to obtain CTAB extract; autoclave the obtained CTAB extract at 121°C for 20min, then add CTAB Mix 2% β-mercaptoethanol and CTAB extract in the volume of the extract, use it as an extraction solvent, and place it in a water bath at 65°C to preheat; 0.5g of the fresh lateral root material of the two-year-old Huangshan Magnolia cultivated in the greenhouse Put it into a mortar pre-cooled by liquid nitrogen, and grind it fully with liquid nitrogen until any granular root system is invisible to the na...
Embodiment 2
[0084] A method for extracting total RNA from the root system of Magnolia huangshan. In the extraction process of this example, except adding LiCl solution in step 3), place it at 4° C. for 16 hours overnight; the others are the same as in Example 1, and the total RNA from the root of huangshan magnolia is finally obtained. Sample 2.
Embodiment 3
[0086] A method for extracting total RNA from the root system of Magnolia huangshan. In the extraction process of this example, step 3) added LiCl solution, and placed at 4°C for 12 hours overnight; the others were the same as in Example 1, and a total RNA sample from the root of huangshan magnolia was finally obtained. 3.
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