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Method for establishment of acute lung injury model of tree shrews and application of model

A technology for acute lung injury and construction methods, applied to medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc., can solve problems such as increasing research difficulty, achieve efficient modeling methods, broaden the research cycle space, The effect of good industrialization prospects

Pending Publication Date: 2020-01-10
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The focus of attention and detection indicators of different diseases are very different; and the differences of different animal species have brought many challenges to the stability of modeling methods and the adaptability of modeling doses, which further increases the difficulty of research

Method used

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  • Method for establishment of acute lung injury model of tree shrews and application of model
  • Method for establishment of acute lung injury model of tree shrews and application of model
  • Method for establishment of acute lung injury model of tree shrews and application of model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Materials and methods:

[0051] 1.1 Experimental materials

[0052] 5 male tree shrews with a body weight of 120-160 g, no internal and external parasites, and normal tree shrews; purchased from Kunming Institute of Zoology, Chinese Academy of Sciences.

[0053] 1.2 Main reagents

[0054] Lipopolysaccharide (LPS) was purchased from Santa Cruz Biotechnology; isoflurane was purchased from Shenzhen Ruiwode Life Technology Co., Ltd.

[0055] 1.3 Preparation of the main solution

[0056] 200mg / kg LPS solution preparation: weigh 26mg of LPS crystals according to the weight of the tree shrew of 130g, and dissolve it in 400μL of PBS (pH=7.35-7.45).

[0057] 1.4 Main Instruments

[0058] Laryngoscope: Simeite 2012-58

[0059] Indwelling needle: BD 20G

[0060] Blood gas needle: BD blood gas needle (2mL)

[0061] Anesthesia machine: 510IP from Ruiwode Life Technology Co., Ltd.

[0062] Micro CT for live imaging of small animals: Super Nova CT (SNC-100)

[0063] Blood ...

Embodiment 2

[0090] 180mg / kg LPS solution preparation: weigh 23.4mg of LPS crystals according to the tree shrew's weight of 130g, and dissolve it in 400μL of PBS.

[0091] Take 2 male tree shrews with no internal and external parasites, normal tree shrews, weighing about 150 g; purchased from Kunming Institute of Zoology, Chinese Academy of Sciences.

[0092] Except that the dose of LPS solution administered by endotracheal intubation was 180 mg / kg, the experimental method and operation of this example were the same as those in Example 1.

[0093] (1) Animals were fasted for 12 hours, and water was not allowed; 180 mg / kg of LPS solution was administered through endotracheal intubation, and the dosage is shown in Table 3.

[0094] Table 3 Dosage

[0095]

[0096] (2) Results: After being induced by LPS, the tree shrews had obvious respiratory symptoms about 3 to 4 days, and their appetite was exhausted; blood gas analysis found that the oxygenation index OI(PaO 2 / FiO 2 ) were 166mmHg...

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Abstract

The invention provides a method for establishment of an acute lung injury model of tree shrews and application of the model. After the tree shrews are narcotized, 180-200 mg / kg of a lipopolysaccharidesolution is intratracheally administrated, and when the lung injury area of the tree shrews reaches a half or more, the acute lung injury animal model of the tree shrews is established. Through the establishment method, lung injury of the tree shrews can reach a half or more after 70 hours; in 96 to 120 hours, the tree shrews clinically show the symptoms of wasting, breathing difficulties and thelike, the lung injury area of the tree shrews is 70% or more, and the blood oxygen index can reach 200 mmHg or below. The modeling method is efficient and high in success rate, and the cost can be effectively saved; the model cycle is 70 hours or more, and the research cycle space for acute lung injury is significantly widened; and the established tree shrew animal model is incapable of self-healing and reversible recovery, the clinical symptoms are more similar to human diseases, and good prospects of industrialization are achieved.

Description

technical field [0001] The invention relates to a modeling method of an animal model, in particular to a method for establishing a tree shrew acute lung injury model and its application. Background technique [0002] Acute lung injury (ALI) is the manifestation of systemic inflammatory response in the lungs. Its clinical manifestations are pulmonary inflammation and increased capillary permeability, which leads to pulmonary edema. It develops into acute respiratory distress syndrome (ARDS), which is pathologically characterized by extensive alveolar damage, manifested as proteinaceous alveolar edema and hypoxemia. Sepsis and multiple trauma are the most common causes of ARDS. The imbalance of inflammatory mediators and anti-inflammatory mediators is a key link in the development of ALI / ARDS. Acute lung injury has always been an urgent problem in critical care medicine and respiratory medicine. The key issue. [0003] The commonly used animal models of acute lung injury inc...

Claims

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Application Information

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IPC IPC(8): A61K31/739A01K67/027
CPCA01K67/027A01K2267/03A61K31/739
Inventor 和君傅江南赵月齐春丽方梅霞宋琳亮邢会杰
Owner JINAN UNIVERSITY
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