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A method for determining isoniazid by quantum dot fluorescence quenching method
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A fluorescence quenching method, isoniazid technology, applied in fluorescence/phosphorescence, measuring device, material analysis by optical means, etc., can solve problems such as unfavorable use, high experimental cost, and complex experimental process of high performance liquid chromatography. , to achieve the effect of simple detection, simple and fast method and low cost
Active Publication Date: 2021-11-12
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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However, the experimental process of high performance liquid chromatography is complicated, and the experimental cost is relatively expensive, which is not conducive to the use of general determination
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Embodiment 1
[0042] Example 1 utilizing Fluorescence Quenching Determination of isoniazid embodiment, the following steps:
[0043] (A) two-step method CdTe QDs synthesized, as follows:
[0044] (1) NaHTe Solution: 10mL of distilled water was added to the vial, after the nitrogen oxygen NaBH 4mmol 4 Reagent bottle is added, until the solid completely dissolved, was added thereto rapidly 1mmol of Te powder, in a nitrogen atmosphere, the reaction at room temperature 2h, to give NaHTe solution.
[0045] (2) Cd precursors: To 250mL three-necked flask was added 100mL of distilled water, stirred at 25 deg.] C under nitrogen, taken 1mmol CdC1 2 · 2.5H 2 O added to the flask and dissolved completely after the addition of 150μL mercaptopropionic acid to this solution, and treated with 1mol·L -1 pH NaOH solution, the solution was adjusted to a value of 10 to 11, to give Cd precursor solution.
[0046] (. 3) CdTe quantum dots: A freshly prepared solution was added rapidly NaHTe three-necked flask equippe...
Embodiment 2
[0067] Example 2 The content of isoniazid in the drug was determined by quantum dot fluorescence quenching. The specific method is as follows:
[0068] Under the optimal conditions of the above experimental four, the content of isoniazid in the commercially available isoniazid is determined, and three different concentrations of isoniazid is prepared according to the amount of the tablets to be tested in the final assay. The indication concentration of isoniazid is 3.646 × 10, respectively. -5 , 1.458 × 10 -4 , 7.292 × 10 -4 Mol / L. The laminated specification of the drug is: 100 mg (each piece), and the distilled water with a distilled water was taken with a distilled water to 1 g / L.
[0069] The specific method is as follows:
[0070] In a 10 ml colorimetric tube, 1 ml of phosphate buffer solution (pH = 5, 0.1 mol / L) was added, 0.5 ml concentration was 8.45 × 10 -7 Mol·L -1 The CDTE quantum dot and a certain amount of isoniazid to be tested, and the concentration of isoniaz...
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Abstract
The invention relates to the application field of nanomaterials, in particular to a method for measuring isoniazid by using a quantum dot fluorescence quenching method. A two-step aqueous phase synthesis of cadmium telluride quantum dots was used for the fluorescence detection of isoniazid content. By investigating the effects of pH value, ionic strength, quantum dot dosage, reaction time and temperature on the reaction system, the results show that the concentration of isoniazid is 2.92×10 ‑5 ~1.02×10 ‑3 When mol / L, the luminescence of quantum dots in the system is obviously suppressed, and there is a good linear relationship, and the detection limit is 4.826×10 ‑7 mol / L, the relative standard deviation was 0.296% (n=10), and the precision was very good. The method established by the invention is simple, fast, low in cost, good in anti-interference ability, and can be directly detected and analyzed. This method not only provides a simple method for detecting isoniazid content, but also broadens the research on the application of quantum dot fluorescence detection methods.
Description
Technical field [0001] The present invention relates to the use of nanomaterials, in particular, to a method for the determination of isoniazid using Fluorescence Quenching Method. Background technique [0002] Isoniazid, scientific name 4-pyridine carboxylic acid hydrazide, also known as isoniazid (isoniazid, 4-Pyridinecarboxylicacid hydrazide, molecular weight 137.14, Acronym INH). Isoniazid is a first-line agents for the treatment of tuberculosis, in particular can play a better therapeutic effect when used with other anti-TB drugs linked. As the mechanism of action of isoniazid treatment of tuberculosis drugs mainly by blocking the synthesis of Mycobacterium tuberculosis bacteria wall acid bacteria, prompting tuberculosis lost the ability to acid dyeability, hydrophobicity, such as the proliferation of death. Also in combination with isoniazid tuberculosis bacteria coenzyme, arresting its corresponding catalysis, so as to achieve a wall tuberculosis bacteria can not be smooth...
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