57 results about "Isonicotinic hydrazide" patented technology

The invention belongs to the field of pharmaceutical chemicals, and particularly relates to a preparation method of topiroxostat. The preparation method disclosed by the invention comprises the following steps: 2-cyanopyridine and formamide are taken as raw materials to react to prepare 2-cyano-4-carbamoyl-pyridine; the 2-cyano-4-carbamoyl-pyridine continues to react with isoniazide to obtain a key intermediate 4-picolinic acid hydrazide-N'-(2-cyanopyridine-4-carbodiimide), and the key intermediate 4-picolinic acid hydrazide-N'-(2-cyanopyridine-4-carbodiimide) is subjected to ring closing to obtain the topiroxostat. The invention provides the novel method for synthesizing topiroxostat, which avoids the use of highly toxic chemical reagents, replaces a traditional catalyst with a green catalyst, is milder in reaction, is economical and environment-friendly, is higher in yield, and is suitable for industrial production.

The invention belongs to the field of application of nano materials and relates to a method for determining isoniazid by using a quantum dot fluorescence quenching method. According to the method of the invention, cadmium telluride quantum dots are synthesized by means of aqueous-phase synthesis through adopting a two-step method so as to be used for the fluorescence detection of isoniazid content. The influence of a pH value, ion strength, a quantum dot dosage, reaction time and temperature on a reaction system is investigated, results show that when the concentration of the isoniazid rangesfrom 2.92*10<-5> to -1.02*10<-3>mol/L, the luminescence of the quantum dots in the system is obviously inhibited; the concentration of the isoniazid and the intensity of the luminescence presents a good linear relationship; the detection limit of the method is 4.826*10<-7>mol/L; the relative standard deviation of the method is 0.296% (n= 10); and the precision of the method is very good. The method provided by the invention has the advantages of simplicity, rapidity, low cost and good anti-interference capability, and can be directly used for detection and analysis. With the method adopted, asimple method for detecting isoniazid content can be realized; and research on the application of a quantum dot fluorescence detection method is expanded.

The invention discloses a benzothiazinone compound, a preparation method thereof and an application of the benzothiazinone compound as an antituberculosis drug, and particularly relates to a novel compound with a benzothiazinone skeleton. The compound has an inhibition effect on tubercle bacillus, especially tubercle bacillus with clinical drug resistance. Results show that the compound shows an obvious antibacterial effect, the antibacterial effect far exceeds that of a positive control isoniazide, and particularly, compared with a positive control pBTZ169, the compound has an obviously goodcLogP value.

The invention provides a preparation method of topiroxostat. The method comprises the following steps: subjecting isonicotinic acid as a starting material to oxidation with hydrogen peroxide to obtainisonicotinic acid-nitric oxide (an intermediate 1); then esterifying with methanol to obtain methyl isonicotinate-nitric oxide (intermediate 2); then performing hydrazinolysis with hydrazine hydrateto obtain isoniazide-nitric oxide (intermediate 3); then reacting with 4-cyanopyridine to obtain an intermediate 4; then cyaniding with trimethylsilyl cyanide to obtain an intermediate 5; and finallyperforming dehydration and cyclization to generate topiroxostat. According to the method, initial raw materials and reagents are cheap and easily available; experimental operation is simple and controllable, extreme reaction conditions are avoided, and the method is suitable for laboratory development and even industrial production; the total yield is high, and the production cost is reduced; purity of the finished product can be ensured.

The invention discloses an isoniazid derivative, a detection reagent and a preparation method, and relates to the technical field of biological detection. An isoniazid immunogen prepared from the isoniazid derivative has high immunogenicity, and an obtained antibody has strong specificity and high titer; an isoniazid enzyme-labeled conjugate in a homogeneous enzyme immunoassay reagent prepared from the derivative is connected with recombinant glucose-6-phosphate dehydrogenase modified by gene engineering; the detection sensitivity is remarkably improved, a sample with the concentration as lowas 5 ng/ml or below can be effectively detected, the specificity is high, and cross reaction with 62 common drugs is avoided; the homogeneous enzyme immunoassay reagent can realize high-throughput andrapid detection of isoniazid content on a full-automatic biochemical analyzer, and is stable in detection result, high in accuracy, simple in detection method and easy to realize, popularize and use.

The invention relates to S-triazolo-thiadiazole and thiadiazine derivatives and an application thereof. Research results show that, the minimal inhibitory concentration (MIC) of the derivatives to mycobacterium tuberculosis standard strains H37Rv is 0.25 [mu]g/mL, and the inhibitory activities of the derivatives to isoniazide/rifampicin-resistant mycobacterium tuberculosis (MDRTB) and rifampicin-resistant mycobacterium tuberculosis (RDRTB) are the same and the MIC of the derivatives to MDRTB and RDRTB is 0.25-4 [mu]g/mL. Meanwhile, the resistance of some derivatives to MDRTB or RDRTB is better than the resistance of the derivatives to reference drugs RIF and INH. At the same time, most derivatives are equivalent in inhibitory activity to Shikimate dehydrogenase, thus being good in development prospect.

The invention discloses a method for preparing medicine-carrying hydroxyapatite/poly glycolide-co-lactide (PLGA)/chitosan demixing microspheres. The method comprises the following steps of: dissolving isoniazide in deionized water, adding hydroxyapatite powder, stirring in a dark place, and freeze-drying to obtain powder; mixing PLGA and the powder uniformly to obtain a hydroxyapatite/PLGA commixed solution containing the isoniazide; dissolving chitosan in an acetic acid aqueous solution to obtain a chitosan solution; mixing the chitosan solution and a polyvinyl alcohol aqueous solution to obtain a chitosan/polyvinyl alcohol solution; and pouring the hydroxyapatite/PLGA commixed solution containing the isoniazide into the chitosan/polyvinyl alcohol solution, stirring under vacuum, washingby using water, and freeze-drying to obtain the medicine-carrying hydroxyapatite/PLGA/chitosan demixing microspheres. The prepared medicine-carrying composite microspheres are regular in spherical shapes, uniform in particle size distribution, high in envelop rate of medicines, long in in-vitro medicine release time and small in burst release; and a preparation process is simple, raw materials are readily available, and industrialization is easy to realize.

The invention discloses a benzothiazinone derivative, a preparation method thereof and application of the benzothiazinone derivative as an antituberculosis drug, which relate to a novel compound witha benzothiazinone skeleton. The compound has an inhibition effect on tubercle bacillus, particularly tubercle bacillus with clinical drug resistance. The benzothiazinone skeleton benzene ring is creatively changed, especially the substituent group is creatively changed to obtain a series of compounds, and an unexpected technical effect is achieved; the compound disclosed by the invention has an excellent inhibition effect on tubercle bacillus; compared with the existing clinical first-line drug isoniazide (MIC 0.5 muM), the reported compound activity has very great advantages, and more importantly, compared with the benzothiazinone antituberculosis drug pBTZ 169 in the existing research stage, the compound provided by the invention has a lower cLogP value and better druggability.

The invention discloses an isoniazid lipid derivate and compositions thereof. The isoniazid lipid derivate is characterized by having an INH-THTT-R structure, wherein INH is isoniazid, THTT is thiadiazine thione, and R is a long fatly chain having 6-20 carbon atoms. The isoniazid lipid derivate can be used for preparing tablets, capsules, injections, aerosols, dry powder inhalers and strong dispersant excipients, wherein the strong dispersant excipients comprise selected liposome, nonionic surface active agent vesicles, particles, nanometer grains, emulsions, self-emulsification systems, micelles and nanometer gel.

The invention discloses a method for adopting isoniazide for preparing nanometer silver sol at room temperature. Silver nitrate is used as a silver source, the isoniazide is adopted to serve as a complexing agent and a reducing agent, a water-soluble surface active agent is used as a protecting agent, the nanometer silver sol with the average grain diameter within 100 nm is prepared at the normal temperature, and particle granularity does not change obviously after the silver sol is placed at the room temperature for 30 days. According to the method, operation at normal temperature and normal pressure can be achieved, the environment is protected, the technological process is simple, and the nanometer silver sol which is wide in silver concentration range, controllable in particle diameter and good in dispersibility is obtained. The adopted isoniazide can have the complexing effect while serving as the reducing agent, the grain size of particles can be effectively controlled, and the stability of the sol is improved.

The invention provides an isoniazide hapten derivative, an isoniazide artificial antigen, a hybridoma cell strain, an isoniazide monoclonal antibody and application, and relates to the technical field of drug monitoring. The isoniazide hapten derivative provided by the invention can realize specific recognition of isoniazide, and the isoniazide antigen obtained by coupling protein by taking-CH2-CH2-CH2-COOH as a coupling arm can accurately obtain an antibody with specific binding capacity to isoniazide. The hybridoma cell strain provided by the invention can generate a high-sensitivity isoniazide monoclonal antibody for equivalent identification of isoniazide, and the antibody has no obvious cross reaction with main metabolites (acetyl isoniazide, isonicotinic acid, nicotinamide and aminosalicylic acid) of isoniazide, and can realize high-sensitivity equivalent identification of isoniazide. Therefore, the compound can be applied to preparation of detection reagents (an isoniazide homogeneous enzyme immunoassay reagent and an isoniazide magnetic particle chemiluminescence detection reagent) for detecting isoniazide, and the clinical detection requirements of isoniazide are met.

The invention relates to the technical field of strain preservation, in particular to a bacterium preservation culture medium and a preparation method thereof. The culture medium comprises the following components: based on 1000ml of the culture medium, 8 to 13 g of beef extract, 2 to 6 g of peptone, 0.2 to 0.5 g of cholate, 0.2 to 0.3 g of aluminum potassium sulfate, 2 to 5 g of malt extract powder, 3 to 7 g of yeast extract powder, 0.3 to 0.6 g of sodium chloride, 5 to 10 g of agar, 0.2 to 0.5 g of soluble ferric salt, 0.01 to 0.05 g of a calcium supplement, 0.02 to 0.05 g of D-cycloserine, 0.001 to 0.004 g of argentein, 0.01 to 0.02 g of vitamin K1, 0.1 to 0.3 g of casein phosphopeptides, 3 to 5 g of glucose, 1 to 2.5 g of lanthanum nitrate, 3 to 5 g of erythritol, 18 to 23 ml of glycerin, 0.1 to 0.2 mg of warfarin sodium tablets, 8 to 12 mg of isoniazide, and 75 to 85 ml of sterile defiberized sheep blood. The formula of the culture medium is rich and comprehensive in nutrient substances, the survival of the strain can be ensured, and the culture medium can still ensure the effectiveness of the strain even if the preservation environment temperature is greatly changed.

The invention discloses a synthesis method of a chelate fiber IAHF and a synthesis method and application of an IAHF-PAR chromatic fiber. The synthesis method of the chelate fiber IAHF comprises the following step: the polyacrylonitrile chelate fiber IAHF is synthesized by taking a polyacrylonitrile fiber as a matrix and isoniazide as a ligand under the condition of a nitrogen protective atmosphere. The synthesis method of the chromatic fiber IAHF-PAR comprises the following steps: putting the polyacrylonitrile chelate fiber IAHF and PAR into a formaldehyde water solution, performing stirring and heating reflux under the condition of a nitrogen protective atmosphere for 2-6 hours, and ending the stirring reaction at 70 DEG C; and performing flushing with warm water, and performing drying to a constant weight to obtain the chromatic fiber IAHF-PAR. The polyacrylonitrile chelate fiber IAHF synthesized by the invention has a heavy metal ion adsorption capability, wherein the selective adsorbability on mercury ions is high, the adsorption capacity is high, and the adsorption rate is high; and the chromatic fiber IAHF-PAR is applicable to detection under different environments, and can be prepared into chromatic materials of different forms.

The invention belongs to the field of analytical chemistry, and particularly relates to a method for determining the content of 4-cyanopyridine and impurities of 4-cyanopyridine in an isoniazide starting material. The measuring method is a gas chromatographic method, and comprises the following specific steps: vaporizing a sample in a vaporization chamber, and introducing the vaporized sample intoa chromatographic column through flowing gas; separating the 4-cyanopyridine SM1 and the impurities of 4-cyanopyridine by means of programmed heating; calculating the content of the impurities by using a principal component self-contrasted method with a correction factor. At present, no reports of simultaneous separation and determination of a plurality of impurities in the SM1 exist. The methodfor measuring the content of the plurality of impurities in the SM1 provided by the invention is simple and feasible, and has high sensitivity and high specificity, so that established quality controlitems of SM1 relevant substances can improve the quality controllability and safety of a product when being applied to control of relevant substances of the product.

The invention discloses a preparation method of isoniazide, which adopts temperature control and proper increase of hydrazine hydrate feeding proportion for reaction, solves the problems of narrow temperature range and difficult operability and controllability of the traditional condensation reaction, widens the reaction temperature to 129-138 DEG C, and has high yield of isoniazide. Meanwhile, refining steps such as decoloration, washing and recrystallization are combined, so that the content of impurities in isoniazid is remarkably reduced, and the requirements of related evaluation work of crude drugs are met. The reaction temperature range is wide, the yield and purity of isoniazide are improved, and a larger selection range is provided for operability and controllability of industrial production of isoniazide.