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Method for determining isoniazid by using quantum dot fluorescence quenching method

A fluorescence quenching method, isoniazid technology, applied in fluorescence/phosphorescence, measurement device, material analysis by optical means, etc., can solve the problems of high experimental cost, complex experimental process of high performance liquid chromatography, unfavorable use and so on , to achieve the effect of simple and rapid method, simple detection and low cost

Active Publication Date: 2020-01-10
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the experimental process of high performance liquid chromatography is complicated, and the experimental cost is relatively expensive, which is not conducive to the use of general determination

Method used

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  • Method for determining isoniazid by using quantum dot fluorescence quenching method
  • Method for determining isoniazid by using quantum dot fluorescence quenching method
  • Method for determining isoniazid by using quantum dot fluorescence quenching method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 A kind of method utilizing quantum dot fluorescence quenching method to measure isoniazid, concrete steps are as follows:

[0043] (1) A two-step method is adopted to synthesize CdTe quantum dots, as follows:

[0044] (1) NaHTe solution: add 10mL of distilled water to the reagent bottle, pass nitrogen gas to deoxygenate, and dissolve 4mmol of NaBH 4 Add it into a reagent bottle, and after the solid dissolves completely, quickly add 1 mmol of Te powder to it, and react for 2 hours under the protection of nitrogen at room temperature to obtain a NaHTe solution.

[0045] (2) Cd precursor: Add 100mL distilled water to a 250mL three-neck flask, stir with nitrogen at 25°C, and take 1mmol CdCl 2 2.5H 2 O was added to the flask, and after the dissolution was complete, 150 μL of mercaptopropionic acid was added to the solution, and 1mol·L -1 The NaOH solution adjusts the pH value of the solution to 10-11 to obtain a Cd precursor solution.

[0046] (3) CdTe quant...

Embodiment 2

[0067] Embodiment 2 Utilizes the quantum dot fluorescence quenching method to measure the content of isoniazid in the medicine, the specific method is as follows:

[0068] Under the optimal conditions of the above-mentioned experiment 4, the content of isoniazid in commercially available isoniazid tablets was determined, and three different concentrations of isoniazid solutions to be tested were prepared according to the labeled amount of the tablets, so that they were included in the final measurement solution. The labeled concentration of isoniazid is 3.646×10 -5 , 1.458×10 -4 , 7.292×10 -4 mol / L. The labeling specification of the medicine is: 100mg (per tablet), take 1 tablet and prepare an isoniazid solution with a stock solution concentration of 1g / L with distilled water.

[0069] The specific method is as follows:

[0070] In a 10mL colorimetric tube, add 1mL phosphate buffer solution (pH=5, 0.1mol / L), 0.5mL concentration is 8.45×10 -7 mol L -1 CdTe quantum dots an...

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Abstract

The invention belongs to the field of application of nano materials and relates to a method for determining isoniazid by using a quantum dot fluorescence quenching method. According to the method of the invention, cadmium telluride quantum dots are synthesized by means of aqueous-phase synthesis through adopting a two-step method so as to be used for the fluorescence detection of isoniazid content. The influence of a pH value, ion strength, a quantum dot dosage, reaction time and temperature on a reaction system is investigated, results show that when the concentration of the isoniazid rangesfrom 2.92*10<-5> to -1.02*10<-3>mol / L, the luminescence of the quantum dots in the system is obviously inhibited; the concentration of the isoniazid and the intensity of the luminescence presents a good linear relationship; the detection limit of the method is 4.826*10<-7>mol / L; the relative standard deviation of the method is 0.296% (n= 10); and the precision of the method is very good. The method provided by the invention has the advantages of simplicity, rapidity, low cost and good anti-interference capability, and can be directly used for detection and analysis. With the method adopted, asimple method for detecting isoniazid content can be realized; and research on the application of a quantum dot fluorescence detection method is expanded.

Description

technical field [0001] The invention relates to the application field of nanomaterials, in particular to a method for determining isoniazid by using a quantum dot fluorescence quenching method. Background technique [0002] Isoniazid, the scientific name is 4-pyridinecarboxylic acid hydrazide, also known as isoniazid (4-Pyridinecarboxylic acid hydrazide, molecular weight is 137.14, abbreviated as INH). Isoniazid is the first-line drug for the treatment of tuberculosis, especially when combined with other anti-tuberculosis drugs, it can exert a better therapeutic effect. The mechanism of action of isoniazid as a drug for the treatment of tuberculosis is mainly by hindering the synthesis of mycolic acid in the bacterial wall of tuberculosis, thereby causing the tuberculosis to lose the ability of acid staining, hydrophobicity, proliferation and other abilities to die. Isoniazid can also combine with the coenzyme of tuberculosis bacteria to prevent its corresponding catalytic ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 察冬梅刘元凤郑孝军何嘉伟
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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