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Virus-based vector compositions useful for transducing eukaryotic cells

一种病毒载体、真核细胞的技术，应用在病毒/噬菌体、逆转录RNA病毒、病毒等方向，能够解决没有考虑到载体颗粒半衰期、高转导效率、不能允许等问题

Pending Publication Date: 2020-01-14
VECTALYS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A major disadvantage in these batches is that they do not allow high transduction of some non-proliferating cells (such as neural cells, macrophages, or hematopoietic stem cells) in a reproducible manner when using low or moderate multiplicity of infection (M.O.I.). conductivity
The main disadvantage of such a harvest time is that it does not take into account the carrier particle half-life

Method used

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  • Virus-based vector compositions useful for transducing eukaryotic cells
  • Virus-based vector compositions useful for transducing eukaryotic cells
  • Virus-based vector compositions useful for transducing eukaryotic cells

Examples

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Embodiment

[0089] The following examples are provided to help better understand the present invention, although the present invention is not limited to these examples.

[0090] Materials and methods

[0091] Plasmid construction. For the production of recombinant virions or recombinant retroviruses, three vectors are used. The first vector provides nucleic acid encoding the viral gag and pol genes ( Figure 2A ). As discussed above, these sequences encode group-specific antigens and reverse transcriptase, respectively (as well as integrase and protease-enzymes necessary for maturation and reverse transcription). The second vector provides nucleic acid encoding the viral envelope (env) ( Figure 2B ). The third vector provides the cis-acting viral sequences required for the life cycle of the virus ( Figure 2C ). This third vector also contains a cloning site for the heterologous nucleic acid sequence to be transferred into the target cell. A schematic diagram of a suitable vector...

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Abstract

The present invention provides viral vector compositions of high titer and purity, as well as methods for production of said compositions. The methods of the invention incorporate multiple features, such as production of viral vector particles in serum free media and multiple harvesting steps following transduction of the producer cell which provides for enhanced production of said viral vectors.The viral vector compositions of the invention, by virtue of their high titer and purity, minimize the deleterious phenotypic changes that typically occur following transduction of target cells, suchas loss of a sub-population of transduced cells, and effects on proliferation, differentiation, reprogramming or functionality of transduced cells.

Description

[0001] This application is a divisional application of the Chinese patent application 201280047202.0 with the filing date of July 26, 2012 and titled "Virus-based vector composition that can be used to transduce eukaryotic cells". [0002] This application claims priority to US Provisional Patent Application Serial No. 61 / 512,289, filed July 27, 2011, the disclosure of which is incorporated herein by reference in its entirety. technical field [0003] The present invention relates to high titer and purity viral vector compositions, methods for producing said compositions, and the use of said viral vector compositions for transduction of eukaryotic cells. The methods of the invention include features, such as production of viral vector particles in serum-free medium, which provide enhanced production of said viral vectors. The viral vector compositions of the present invention, due to their high titer and purity, usually lead to deleterious phenotypic changes after target cell ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/02C12N15/867
CPCC12N2740/15051C12N7/02
Inventor P.布耶H.韦尼奥R.加永Y.莫阿尔
Owner VECTALYS