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Detection method for interaction of graphene-nano silver compound and double-stranded DNA (deoxyribonucleic acid)

A detection method and nano-silver technology, which can be used in material excitation analysis, fluorescence/phosphorescence, etc., can solve problems such as detection of the interaction between difficult graphene-nano-silver complexes and double-stranded DNA.

Active Publication Date: 2020-01-17
JINING MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Existing technology, it is difficult to carry out comprehensive and scientific detection on the interaction between graphene-nanosilver composite and double-stranded DNA, there are certain defects, so it needs to be improved

Method used

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  • Detection method for interaction of graphene-nano silver compound and double-stranded DNA (deoxyribonucleic acid)
  • Detection method for interaction of graphene-nano silver compound and double-stranded DNA (deoxyribonucleic acid)
  • Detection method for interaction of graphene-nano silver compound and double-stranded DNA (deoxyribonucleic acid)

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Embodiment 1

[0029] A detection method for the interaction between a graphene-nanometer silver complex and double-stranded DNA, specifically comprising the steps of:

[0030] S1, material preparation: use the chemical reduction method to synthesize graphene-nanometer silver composites, the specific operation is: take 25ml of graphene oxide with a concentration of 1mg / ml after dialysis, configure silver ammonia solution, and mix 52.5mgAgNO 3 Dissolve in 2.5ml of deionized water, and drop 3% ammonia water into the silver nitrate aqueous solution until the precipitate just disappears, stir and mix the newly prepared silver ammonia solution and graphene oxide aqueous solution at 50°C for 30min, then add 25ml containing 0.5g of glucose in aqueous solution, the mixed solution was heated up to 95°C, and the mixed solution was stirred and reacted at 95°C for 1h. After the reaction solution was naturally cooled, the product was centrifuged and washed three times with ethanol and deionized water resp...

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Abstract

The invention discloses a detection method for interaction of a graphene-nano silver compound and double-stranded DNA (deoxyribonucleic acid). The detection method can be used for comprehensively detecting the interaction of the graphene-nano silver compound and the double-stranded DNA through ultraviolet spectrometry, fluorescence spectrometry and variable-temperature fluorescence measurement, and has important significance for exploring the application of the graphene-nano silver compound in the aspect of detecting the double-stranded DNA.

Description

technical field [0001] The invention belongs to the technical field of graphene-nano-silver, and more specifically relates to a detection method for the interaction between a graphene-nano-silver complex and double-stranded DNA. Background technique [0002] Biomacromolecules are the basic substances that make up living organisms, mainly including proteins and nucleic acids. These macromolecules have extremely important physiological functions for the growth and development of living organisms, such as transmitting genetic information, performing metabolism, promoting growth and development, and providing life energy. Deoxyribonucleic acid is the carrier of genetic genes. It has the ability to store and transmit information, and is the carrier of genetic information and the material basis of gene expression. It can be divided into single-stranded DNA and double-stranded DNA. Single-stranded DNA is different from double-stranded DNA in terms of molecular hydrodynamic propert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 徐香玉杜中玉杨林青毛旭艳刘君
Owner JINING MEDICAL UNIV