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Nucleic acid double detection test strip for brucella and mycobacterium tuberculosis

A technology for Brucella and Mycobacterium tuberculosis, which is applied in the field of nucleic acid double detection test strips for Brucella and Mycobacterium tuberculosis, can solve the problems such as the detection of Brucella and Mycobacterium tuberculosis, and the inability to perform pathogen detection methods at the same time.

Pending Publication Date: 2020-01-21
ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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  • Abstract
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Problems solved by technology

[0005] In order to solve the problem that the existing pathogen detection method cannot simultaneously detect Brucella and Mycobacterium tuberculosis, the present invention provides a nucleic acid dual detection test strip for Brucella and Mycobacterium tuberculosis

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  • Nucleic acid double detection test strip for brucella and mycobacterium tuberculosis
  • Nucleic acid double detection test strip for brucella and mycobacterium tuberculosis
  • Nucleic acid double detection test strip for brucella and mycobacterium tuberculosis

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Embodiment Construction

[0021] The nucleic acid double detection test strip for Brucella and Mycobacterium tuberculosis of the present invention is mainly characterized in that the primers and / or probes are labeled with biotin and / or fluorescent groups, and a layer with two kinds of labels is used to label the primers and / or probes. Rapid detection of nucleic acid amplification products using analytical test strips. Wherein, the nucleic acid amplification product is obtained by amplifying specific fragments of Brucella and Mycobacterium tuberculosis.

[0022] A nucleic acid double detection test strip for Brucella and Mycobacterium tuberculosis of the present invention mainly includes:

[0023] Brucella forward primer sequence F1: catccttacgcgcaacgatatggatcgtttcc.

[0024] Brucella reverse primer sequence R1: biotin-tttccggcgcaggcgaaaagggcgtgccgggtctc.

[0025] Brucella probe sequence Probe1: FAM (or FITC)-accttgcccttgccatcataaaggccggtgc(dSpacer)gttataggcccaataggc-P.

[0026] Mycobacterium tubercu...

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Abstract

The invention discloses a nucleic acid double detection test strip for brucella and mycobacterium tuberculosis and relates to the field of bacterial detection. The nucleic acid double detection test strip comprises a brucella forward primer sequence shown in SEQ ID NO:1, a brucella reverse primer sequence shown in SEQ ID NO:2, a brucella probe sequence shown in SEQ ID NO:3, a mycobacterium tuberculosis forward primer sequence shown in SEQ ID NO:4, a mycobacterium tuberculosis reverse primer sequence shown in SEQ ID NO:5 and a mycobacterium tuberculosis probe sequence shown in SEQ ID NO:6. Isothermal amplification is performed on the brucella and the mycobacterium tuberculosis separately by a fluorescence marking method, nucleic acid detection is performed in combination with an immunochromatography technology, the method is more accurate than a traditional serologic detection method, and besides, the method can distinguish infection with the brucella and mycobacterium tuberculosis of animals simultaneously and has great significance in clinical control of brucella and mycobacterium tuberculosis.

Description

technical field [0001] The invention relates to the technical field of bacterial detection, in particular to a nucleic acid double detection test strip for Brucella and Mycobacterium tuberculosis. [0002] technical background [0003] Both brucellosis and tuberculosis are zoonotic infectious diseases, and are currently classified as second-class animal diseases by the state. With the improvement of people's living standards and the adjustment of dietary structure, dairy cattle and sheep raising and other breeding industries have developed rapidly, and the chances of Brucella bovis and Mycobacterium tuberculosis infecting humans have also increased. The disease not only affects the development of aquaculture, but also seriously threatens the health and safety of the people and the health of the people. Therefore, the prevention and detection of brucellosis and tuberculosis have attracted more and more attention. [0004] At present, bacterial detection is the most accurate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6844C12Q1/04
CPCC12Q1/689C12Q1/6844C12Q2531/119C12Q2563/107C12Q2565/625
Inventor 郎需龙卜昭阳祝令伟
Owner ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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