Establishment and application method of prediction model for evaluating safety of lactobacillus fermented food
A lactic acid bacteria fermentation and predictive model technology, applied in biochemical equipment and methods, microbial measurement/inspection, instruments, etc., can solve the problems of lack of evaluation methods for microbial safety, time-consuming, high cost, etc., and achieve artificial intelligence supervision Management, accurate assessment of security, cost-saving effects
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[0051] Example 1 Application of the method of the present invention in the safety assessment of fermented salmon products
[0052] 1. Establishment of predictive model
[0053] 1. Obtain the maximum concentration N of starter lactic acid bacteria and food-borne pathogenic bacteria in salmon to be fermented through preliminary experiments max and the theoretical minimum growth temperature T min .
[0054] Lactic acid bacteria and food-borne pathogenic bacteria were inoculated in the salmon to be fermented and cultured at a certain temperature; the fermented salmon samples were quantitatively taken out at different culture times, diluted and spread on the selective plate corresponding to each bacteria, Count the colonies after a single colony grows to obtain the concentration N of the strains at different culture times t ; Use formula (A) to carry out the fitting of growth curve (operate by Microsoft Excel and programming solution add-in), obtain respectively the maximum conc...
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