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Paraffin Barrier Coatings for Microscope Slides

A technology for microscope slides and coatings, applied in microscopes, special-purpose bioreactors/fermenters, enzymology/microbiology devices, etc., can solve problems that do not support direct encapsulation of biological materials or IHC processing

Active Publication Date: 2021-11-05
深圳市诺高实验器材有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such embodiments do not support direct encapsulation of biological material or IHC processing, especially deparaffinization of tissue sections

Method used

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  • Paraffin Barrier Coatings for Microscope Slides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Spray application method:

[0040] Spray on surface with low air flow. Low liquid to air mixing is preferred. The mixture is sprayed onto slides through a mask to coat the PRS targets. Typically 1-2 passes are required to form a layer with a thickness less than 5 microns. Both the paraffin mixture reservoir and the spray head are heated to slightly above 56°C to ensure that the paraffin is liquid and remains liquid during the flight from the spray head to the slide. The spray coverage from the sprinkler head is approximately 0.375 inches, but a mask can also be used for smaller masked areas. Post-infrared reheating ensures a 100% airtight seal.

Embodiment 2

[0042] Screen printing method:

[0043] A stainless steel wire mesh is heated by passing an electric current through the wire mesh between two parallel sides. The temperature of the screen needs to be slightly below the melting temperature of the paraffin so that the paraffin does not penetrate to the underside of the screen. Basically, paraffin behaves more like a paste than a liquid. PRS needs to be reheated to ensure a 100% seal.

Embodiment 3

[0045] Inkjet method:

[0046] Inkjet heads require an integrated heater within the print head to keep the paraffin in a liquid state. A post heat cycle on the slide will ensure a 100% seal.

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Abstract

Provides barrier coating on microscope slides. In particular, an optional application of a paraffin layer to shield biological material and inorganic chemical deposits from microbial attack and oxidation is provided. More specifically, there is also provided the application of a paraffin layer on a microscope slide as an exposure shield for reactive targets of deposited biomaterial. The paraffin mask prevents exposure of biological material and chemical targets that could lead to degradation due to oxidation, and provides resistance to fungal growth while using existing staining processing steps to remove paraffin from the mask and co-existing tissue sections cleared.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Application No. 62 / 520169, filed June 15, 2017, and U.S. Provisional Application No. 62 / 520319, filed June 15, 2017, the disclosure of each of which is incorporated by reference in its entirety this. technical field [0003] This invention relates to barrier coatings on microscope slides. In particular, the present invention relates to the application of a paraffin layer to shield biological material and inorganic chemical deposits from microbial attack and oxidation. More particularly, the present invention relates to providing a layer of paraffin wax applied to a microscope slide as an exposure shield for reactive targets of deposited biological material. The paraffin mask prevents exposure of biological material and chemical targets, which can lead to degradation due to oxidation, and provides resistance to fungal growth, while using existing staining processin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/34G02B21/00C12M1/00
CPCG02B21/34G02B1/14G01N33/543
Inventor 弗雷德里克·克努特·哈舍岑子祥
Owner 深圳市诺高实验器材有限公司