Photocuring enzyme cross-linking bioink, preparation method of bioink and 3D printing method

A bio-ink and 3D printing technology, applied in the field of bio-3D printing, can solve the problem of single cross-linking method, 3D bionic structure is difficult to take into account cell compatibility, mechanical strength and degradability, single component of bio-3D printing ink, etc. problem, to achieve the effect of high cytocompatibility, high degradability and high cell adhesion
CN110743041AInactive Publication Date: 2020-02-04SOUTHERN MEDICAL UNIVERSITY
4 Cites 9 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTHERN MEDICAL UNIVERSITY
Publication Date
2020-02-04
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses photocuring enzyme cross-linking bioink, a preparation method of the bioink and a 3D (three dimensional) printing method. The photocuring enzyme cross-linking bioink comprisesthe following ingredients: 0.1-50wt% of fibrinogen, 0.1-50wt% of methacrylic anhydride modified gelatin and 0.01-20wt% of photoinitiator. The photocuring enzyme cross-linking bioink has diversified material components; enzyme cross-linking of the fibrinogen is added based on ultraviolet light cross-linking of methacrylic anhydride modified gelatin; and a double-network hydrogel system comprising bioactive cells is constructed. A bionic structure printed by the photocuring enzyme cross-linking bioink has the characteristics of high cell compatibility, high mechanical strength, high degradability and no cytotoxicity, provides a good bionic environment for proliferation, differentiation and protraction of the bioactive cells and is applicable to the field of tissue engineering such as biological tissue construction and repair.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of biological 3D printing, in particular to a photocurable enzyme cross-linked bio-ink, a preparation method thereof and a 3D printing method. Background technique

[0002] As one of the preparation methods of biomimetic structures, 3D bioprinting technology has the advantages of precisely controlling the morphology and cell distribution of biomimetic structures. Bio-3D printing technology can precisely control the printing structure in three-dimensional space, and can print complex bionic structures according to the unique anatomical and physiological standards of the human body, providing a new technical means for the medical field of tissue engineering and clinical repair of tissue or organ defects.

[0003] Existing bio-3D printing inks have a single component and usually only contain a hydrogel system. The cross-linking method is also relatively simple, and usually only one of chemical cross-linking, p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More