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No-microspheres homogeneous luminescent system of biotin-avidin or streptavidin

A homogeneous chemiluminescence and streptavidin technology, applied in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, scientific instruments, etc., to achieve the effect of improving detection sensitivity and improving chemiluminescence efficiency

Active Publication Date: 2020-02-11
无锡壹闪生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no microsphere-free homogeneous chemiluminescence system based on biotin-avidin or biotin-streptavidin, which can be used in autoimmune homogeneous chemiluminescence systems, POCT and microfluidic chips, etc. quantitative analysis

Method used

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  • No-microspheres homogeneous luminescent system of biotin-avidin or streptavidin
  • No-microspheres homogeneous luminescent system of biotin-avidin or streptavidin
  • No-microspheres homogeneous luminescent system of biotin-avidin or streptavidin

Examples

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Embodiment 1

[0035] Detection of S100B protein by NMHL technology

[0036] A method for quantitatively measuring S100B protein based on a biotin-streptavidin-free microsphere homogeneous chemiluminescent system, the method steps are as follows: S1, reagent preparation: prepare calibrator, auxiliary agent and trigger agent respectively, Preparation of biotin-labeled antibodies or antigens, 9,10-dihydroacridine-labeled antibodies or antigens, HRP-labeled avidin or streptavidin; S2, adding samples: adding enzyme labels to calibrators or samples substances, biotin markers, and luminescent markers, oscillated and mixed to prepare the test mixture A; S3, detection: add auxiliary agents to the test mixture A, oscillate and mix, let stand, add triggers, oscillate and mix , prepare the mixture B to be tested, perform detection, and read the luminescence value; S4, calculation: perform logistic four-parameter fitting on the concentration and luminescence value of the calibrator, and calculate the sa...

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Abstract

The invention discloses a no-microspheres homogeneous chemiluminiscent system of biotin-avidin or streptavidin as well as a method for applying the system to quantitative antigen or antibody measurement, and relates to the technical field of chemiluminiscent systems. The no-microspheres homogeneous chemiluminiscence system comprises a biotin-labeled antibody or antigen, a 9, 10-dihydracridine-labeled antibody or antigen, and HRP-labeled avidin or streptavidin. According to the system, a homogeneous system without microspheres is used; the biotin-avidin or streptavidin system is used to greatlyimprove the chemiluminiscence efficiency and detection sensitivity, the lowest detection concentration of the example S100B protein can reach 0.015 ng / ml; and a detection process of the system does not need cleaning, is convenient and simple, and can be used to an automatic immune homogeneous chemiluminescence system, POCT, a micro-fluidic chip and the like.

Description

technical field [0001] The invention relates to the technical field of chemiluminescence systems, in particular to a biotin-avidin or streptavidin-free homogeneous chemiluminescence system and a method for quantitatively measuring antigens or antibodies. Background technique [0002] LOCI (Luminescent oxygen channeling immunoassay) diagnostic technology was discovered by American scientist Professor Ullman in the early 1990s and developed by Deling Corporation of the United States. This technology is based on the short-distance diffusion of two nanometer microspheres using single-line oxygen energy to stimulate the chemiluminescent reaction of the adjacent sites that have been formed to measure the interaction between biomolecules. It is a non-radioactive detection analysis. In this method, by the close binding of biomolecules on capture microspheres, energy transfer occurs from one microsphere to another, through chemical reactions and finally to generate a luminescent sign...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/536G01N33/532
CPCG01N21/76G01N33/536G01N33/532
Inventor 奚伟红
Owner 无锡壹闪生物科技有限公司
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