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Biotin-avidin or streptavidin-free homogeneous chemiluminescent system

A homogeneous chemiluminescence and streptavidin technology, applied in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, scientific instruments, etc., to achieve the effect of improving chemiluminescence efficiency and detection sensitivity

Active Publication Date: 2022-05-17
无锡壹闪生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0007] At present, there is no microsphere-free homogeneous chemiluminescence system based on biotin-avidin or biotin-streptavidin, which can be used in autoimmune homogeneous chemiluminescence systems, POCT and microfluidic chips, etc. quantitative analysis

Method used

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  • Biotin-avidin or streptavidin-free homogeneous chemiluminescent system
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  • Biotin-avidin or streptavidin-free homogeneous chemiluminescent system

Examples

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Embodiment 1

[0035] Detection of S100B protein by NMHL technology

[0036] A method for quantitatively measuring S100B protein based on a biotin-streptavidin-free microsphere homogeneous chemiluminescent system, the method steps are as follows: S1, reagent preparation: prepare calibrator, auxiliary agent and trigger agent respectively, Preparation of biotin-labeled antibodies or antigens, 9,10-dihydroacridine-labeled antibodies or antigens, HRP-labeled avidin or streptavidin; S2, adding samples: adding enzyme labels to calibrators or samples substances, biotin markers, and luminescent markers, oscillated and mixed to prepare the test mixture A; S3, detection: add auxiliary agents to the test mixture A, oscillate and mix, let stand, add triggers, oscillate and mix , prepare the mixture B to be tested, perform detection, and read the luminescence value; S4, calculation: perform logistic four-parameter fitting on the concentration and luminescence value of the calibrator, and calculate the sa...

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Abstract

The invention discloses a microsphere-free homogeneous chemiluminescence system of biotin-avidin or streptavidin, and a method for quantitatively measuring antigens or antibodies, and relates to the technical field of chemiluminescence systems. The microsphere homogeneous chemiluminescence system includes: biotin-labeled antibody or antigen, 9,10-dihydroacridine-labeled antibody or antigen, HRP-labeled avidin or streptavidin. Biotin-avidin or biotin-streptavidin No Microspheres Honogeneous Luminescent (Biotin-Avidin / streptavidin) system does not use microspheres, it is a homogeneous system; the introduction of biotin ‑Avidin / streptavidin system can greatly improve chemiluminescence efficiency and detection sensitivity. The minimum detection concentration of S100B protein in the example can reach 0.015ng / ml; the detection process of this system does not need cleaning, is convenient and simple, and can be used in automatic Immunohomogeneous chemiluminescence system, POCT and microfluidic chips, etc. for rapid quantitative analysis.

Description

technical field [0001] The invention relates to the technical field of chemiluminescence systems, in particular to a biotin-avidin or streptavidin-free homogeneous chemiluminescence system and a method for quantitatively measuring antigens or antibodies. Background technique [0002] LOCI (Luminescent oxygen channeling immunoassay) diagnostic technology was discovered by American scientist Professor Ullman in the early 1990s and developed by Deling Corporation of the United States. This technology is based on the short-distance diffusion of two nanometer microspheres using single-line oxygen energy to stimulate the chemiluminescent reaction of the adjacent sites that have been formed to measure the interaction between biomolecules. It is a non-radioactive detection analysis. In this method, by the close binding of biomolecules on capture microspheres, energy transfer occurs from one microsphere to another, through chemical reactions and finally to generate a luminescent sign...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N33/536G01N33/532
CPCG01N21/76G01N33/536G01N33/532
Inventor 奚伟红
Owner 无锡壹闪生物科技有限公司
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