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A culture medium and detection method for detecting bifidobacteria

A technology of bifidobacteria and detection method, applied in the field of microbial detection, can solve problems such as complex medium formula, and achieve the effect of being conducive to detection and proliferation promotion

Active Publication Date: 2020-05-26
BIOSTIME GUANGZHOU HEALTH PROD
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Problems solved by technology

However, this medium has the characteristics of complex formula

Method used

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  • A culture medium and detection method for detecting bifidobacteria

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Embodiment 1

[0029] 1. Equipment and materials

[0030] In addition to routine sterilization and culture equipment in the microbiology laboratory, other equipment and materials are as follows:

[0031] 1.1 Constant temperature incubator: 36℃±1℃;

[0032] 1.2 Homogenizer and aseptic homogenizing bag, homogenizing cup or sterilized mortar;

[0033] 1.3 Refrigerator: 2℃~5℃;

[0034] 1.4 Balance: Sensitivity 0.1g;

[0035] 1.5 Sterile test tube: 18mm×180mm, 15mm×100mm;

[0036] 1.6 Sterile pipette: 1mL (with 0.01mL scale), 10mL (with 0.1mL scale)

[0037] 1.7 Spiral inoculator or L-shaped glass smear rod

[0038] 1.8 Anaerobic incubator box or anaerobic tank and supporting consumables

[0039] 1.9 Vortex shaker

[0040] 2. Media and reagents

[0041] 2.1 Soy peptone

[0042] 2.2 MRS medium

[0043] 2.3 Estradiol benzoate and ethinyl estradiol: chemically pure

[0044] 2.4 Dipotassium hydrogen phosphate: analytically pure

[0045] 2.5 Potassium dihydrogen phosphate: analytically pur...

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Abstract

The invention relates to a culture medium for detecting bifidobacterium and a detection method thereof, the culture medium for detecting bifidobacterium is prepared by adding estrogen into a lactic acid bacteria culture medium, and the concentration of estrogen in the culture medium is 1-10ppm. The estrogen with a certain concentration is added into the lactic acid bacteria culture medium, so thatthe proliferation of other lactic acid bacteria is inhibited, the proliferation of bifidobacteria is promoted, and the detection of bifidobacteria is facilitated.

Description

technical field [0001] The invention belongs to the technical field of microbial detection, in particular to a culture medium and a detection method for detecting bifidobacteria, in particular to a culture medium and a detection method for detecting bifidobacteria in compound lactic acid bacteria. Background technique [0002] Lactic acid bacteria (LAB) is a general term for bacteria that can produce large amounts of lactic acid from fermentable carbohydrates. This kind of bacteria is widely distributed in nature and has rich species diversity, including at least 18 genera and more than 200 species in total. Except for a very few, most of them are essential flora in the human body and have important physiological functions, and widely exist in the intestinal tract of the human body. [0003] Lactic acid bacteria are not only ideal materials for the study of classification, biochemistry, genetics, molecular biology and genetic engineering (with important academic value in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12N1/38C12N1/20C12R1/01
CPCC12N1/20C12N1/38C12Q1/045G01N2333/195
Inventor 刘斐童马博轩黄惠英李爱媚陈桔淳胡瑞标林媛邓超明
Owner BIOSTIME GUANGZHOU HEALTH PROD
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