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Molecular markers associated with goat litter size and growth traits and their applications

A technology for growth traits and molecular markers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.

Active Publication Date: 2020-08-21
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no relevant report on the study of goat ZBP1 gene as a molecular marker for goat litter size and growth traits

Method used

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  • Molecular markers associated with goat litter size and growth traits and their applications
  • Molecular markers associated with goat litter size and growth traits and their applications
  • Molecular markers associated with goat litter size and growth traits and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Obtaining SNP Detection Fragment of Goat ZBP1 Gene and Establishment of Polymorphism Site Detection Method

[0022] 1. Extraction of Goat Genomic DNA

[0023] The test goat breeds of the present invention are Boer goats, Macheng black goats and black-headed goats (hybrid breeds of Boer goats and Macheng black goats), and the samples are from the breeding sheep farm of the Institute of Animal Husbandry and Veterinary Medicine, Hubei Academy of Agricultural Sciences. The goat genomic DNA was extracted using the Blood Genomic DNA Extraction Kit (Product No.: DP348) produced by Beijing Tiangen Biochemical Technology Co., Ltd., and the specific steps were referred to the kit instructions. The concentration and quality of the extracted DNA were tested and stored at -40°C for future use.

[0024] 2. Obtaining the detection fragment of goat ZBP1 gene SNP genetic marker

[0025] (1) PCR amplification

[0026] According to the SNP genetic marker detection sequence (a...

Embodiment 2

[0039] Example 2 Detection of polymorphism distribution of molecular markers prepared by the present invention in goat populations

[0040] In this example, the polymorphism of the g.7919G>A site in the seventh exon of the goat ZBP1 gene was detected in the populations of Boer goats, black-headed goats and Macheng black goats, and the detection results are shown in Table 3.

[0041] Table 3-Genotype frequency and allele frequency of goat g.7919G>A locus in goat population

[0042]

[0043] From the results in Table 3, it can be seen that the g.7919G>A locus has three genotypes in the Boer goat, black-headed goat and Macheng black goat populations, among which the homozygous genotype is dominant, and the AA genotype has a low proportion . The dominant alleles in the Boer goat, Black-head sheep and Macheng black goat populations were consistent, and the allele G of the g.7919G>A locus was the dominant allele in the three goat populations.

Embodiment 3

[0044] Example 3 Correlation analysis and application of goat g.7919G>A molecular markers prepared by the present invention and litter size traits

[0045] In order to determine whether the g.7919G>A site in the seventh exon of the goat ZBP1 gene is related to the difference in litter size traits of Boer goats, black-headed goats and Macheng black goats, the method established in Example 1 was used for polymorphism detection. The correlation between the three genotypes of goat g.7919G>A locus and the litter size traits of goats was analyzed. SAS statistical software (SASInstitute Inc, Version 9.1) GLM program was used to conduct variance analysis of different SNP genotype combinations, and a significance test was performed. The model used was:

[0046] Y ijklm =μ+P i +S j +F k +G l +M m +e ijklm ;

[0047] Y ijklm is the trait phenotype value, μ is the mean value, P i is the influence of parity i (i=1, 2, 3, 4), S j is genotype effect (including gene additive effect...

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Abstract

The invention provides a molecular marker associated with a litter size and a growth character of a goat. The molecular marker is located in a partial DNA (deoxyribonucleic acid) sequence of a seventhexon of a goat ZBP1 gene; a nucleotide sequence of the molecular marker is shown as SEQ ID NO. (sequence identifier number) 1 and is 321bp in length; and G>A base mutation exists at 173bp of the sequence. The invention further provides a detection kit of SNP (single nucleotide polymorphism) associated with the litter size and the growth character. The detection kit comprises a primer pair shown as SEQ ID NO. 2-3, and an SNaPshot single base extension primer shown as SEQ ID NO. 4. The novel molecular marker is explored and associated with two characters (the litter size and the growth character of the goat); early selection of the litter size and the growth character of the goat is achieved; and a detection method is quick and accurate and is independent of a breeding environment conditionfactor.

Description

technical field [0001] The invention relates to the technical field of goat molecular marker screening, in particular to molecular markers associated with goat litter size and growth traits and applications thereof. Background technique [0002] Lambing traits and growth traits are the most important economic traits of goats, which are of great significance to the development of goat industry. Improving the litter size and body size traits of goats is an important measure to improve the economic benefits of production for goats. Research using genomics methods can screen out candidate genes and molecular markers related to important economic traits of goats at the level of the entire genome, enabling researchers to better understand the genetic mechanism of goat traits. It is difficult to obtain greater genetic progress in a short period of time through conventional breeding methods. With the continuous development of applied genomics, molecular biology and molecular geneti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2533/101
Inventor 陶虎陈明新张年杨娟熊琪李晓锋索效军杨前平刘洋田宏张鹤山熊军波张凤
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI