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Metabolic marker of medulloblastoma in urine and application thereof

A technology of medulloblastoma and metabolites, which is applied in the field of metabolic markers of medulloblastoma in urine and its early diagnosis and postoperative condition monitoring

Inactive Publication Date: 2020-02-14
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, studies on humoral markers of MB are lacking

Method used

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  • Metabolic marker of medulloblastoma in urine and application thereof
  • Metabolic marker of medulloblastoma in urine and application thereof
  • Metabolic marker of medulloblastoma in urine and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Example 1: Research on Early Specific Diagnostic Markers of Medulloblastoma

[0045] Below in conjunction with accompanying drawing, further illustrate the present invention through the embodiment, but not as limitation to the present invention. Specific materials and their sources used in embodiments of the invention are provided below. However, it should be understood that these are merely exemplary and not intended to limit the present invention, and materials identical or similar to the type, model, quality, property or function of the following reagents and instruments can be used to implement the present invention. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0046] We used liquid chromatography-high resolution mass spectrometry (LC-MS) to detect metabolites in urine throug...

Embodiment 2

[0078] Example 2: Research on markers for disease monitoring of medulloblastoma after surgery

[0079] Materials and Reagents

[0080] 1) Instrument: Waters H-class liquid chromatograph (waters company) LTQ-Orbitrap velos pro mass spectrometer (Thermofisher Scientific company).

[0081] 2) Main reagents: acetonitrile (Thermofisher Scientific Company); C18 reverse-phase chromatographic column (3.0 mm×100 mm, C18, 1.7 μm, Waters Company).

[0082] 3) Samples: preoperative urine samples of 112 patients (67 males, 45 females) with medulloblastoma, urine samples of 80 patients (51 males, 29 females) with medulloblastoma one month after operation Fluid samples; urine from 118 age- and gender-matched normal control groups (67 males and 51 females); from Beijing Tiantan Hospital. The average age of each group is not more than 2 years old.

[0083] 2.1 Collection of human urine samples

[0084] Collect fasting morning urine and centrifuge at 5000g for 30min to remove sediment.

[...

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Abstract

The invention relates to a metabolic marker of medulloblastoma in urine and an application thereof. Specifically, the invention relates to an application of a reagent in preparing kits and / or chips for diagnosis and / or prognostic of medulloblastoma for detecting metabolites levels in urine of a subject. Metabolites are selected from one or more of the following matters: Uropterin, 20-oxo-leukotriene E4, Cortolone, Tetrahydrocortisone, [6]-Gingerdiol 3,5-diacetate, Pteroside Z, 12-oxo-2,3-dinor-10,15-phytodienoic acid, Melleolide M, Ethyl 7-epi-12-hydroxyjasmonate glucoside, Piperdial, Epishyobunone, 4R,5S,7R,11S)-11,12-Dihydroxy-1(10)-spirovetiven-2-one11-glucoside, PC-M5 and Acuminoside.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a metabolic marker of medulloblastoma in urine and its use in early diagnosis and postoperative condition monitoring. Background technique [0002] Medulloblastoma (MB) is the most common malignant central nervous system tumor in children (see Brandes, A.A. and M.K.Paris, Review of the prognostic factors inmedulloblastoma of children and adults. Crit Rev Oncol Hematol, 2004.50(2): p.121-8; Saran, F., Recent advances in pediatric neuro-oncology. Curr OpinNeurol, 2002.15(6): p.671-7), accounting for 8% to 10% of children's brain tumors (see Archer, T.C., E.L. Mahoney, and S.L. Pomeroy, Medulloblastoma: Molecular Classification-Based Personal Therapeutics. Neurotherapeutics, 2017.14(2):p.265-273). Early detection and early treatment can effectively improve the five-year survival rate of patients (see Ramaswamy, V. et al., Recurrence patterns across medulloblastoma subgr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/7266G01N2030/027
Inventor 孙伟刘晓燕李京郭正光孙海丹汤晓月
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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