Cerebrospinal fluid VGF protein kit and application thereof in medulloblastoma metastasis evaluation

Abstract

The invention belongs to the technical field of biology, and relates to a tumor diagnosis kit, in particular to a cerebrospinal fluid VGF protein kit and application of the cerebrospinal fluid VGF protein kit to detection and pre-judgment of myeloblastoma metastasis. The kit comprising mouse anti-human VGF biotin labeled monoclonal antibody, streptomycin antibiotic protein peroxidase working fluidand other components is designed, then a collected cerebrospinal fluid sample is detected through an ELISA method, corresponding diagnosis is conducted according to the concentration of VGF in cerebrospinal fluid, and when the concentration of VGF is reduced to one third of the concentration of VGF after an operation, it is preliminarily judged that myeloblastoma cerebrospinal fluid metastasizes.The method disclosed by the invention is applied to diagnosis of myeloblastoma prediction, can be used for screening high-risk groups and guiding tumor staging, and provides a basis for searching sensitive and specific myeloblastoma cerebrospinal fluid markers.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a tumor diagnosis kit, in particular to a cerebrospinal fluid VGF protein kit and its application in evaluating medulloblastoma metastasis. The kit detects the VGF protein in the cerebrospinal fluid, and provides a basis for searching for sensitive and specific cerebrospinal fluid markers of medulloblastoma metastasis. Background technique [0002] The prior art discloses that medulloblastoma is the most common malignant brain tumor in children, accounting for about 20% of children's tumors. The prognosis of this disease in adults is also very poor. Medulloblastoma has become a major brain tumor that endangers people's health. disease. In 2016, WHO classified medulloblastoma into different molecular subtypes: SHH, WNT, GROUP3, and GROUP4. The current standard treatment for medulloblastoma is: surgical resection + postoperative radiotherapy + postoperative chemotherapy. The 5-...

AI Technical Summary

Problems solved by technology

Facts have proved that the ionization degree of different peptides and the response of different ions in the mass spectrometer are not the same, so the use of information of each peptide in traditional mass spectrometry to deduce the content of the corresponding protein may lead to a decrease in the stability and sensitivity of quantitative analysis. The consequence is that some differential proteins cannot be found

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