Enteric microorganism detection system based on micro-droplets

A technology of intestinal microbiology and detection system, which is applied in the direction of microbiological measurement/inspection, enzymology/microbiology device, specific-purpose bioreactor/fermentation tank, etc., and can solve the problems such as the final size adjustment of difficult multi-encapsulation droplets

Pending Publication Date: 2020-02-18
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Therefore, the current multiple emulsion preparation chips are usually dedicated chips, which can only prepare multiple-encapsulated droplets of a specific size, and it is difficult to flexibly adjust the final size of the multiple-encapsulated droplets.
In the actual detection process, due to the complexity and variability of the substances to be tested, there is an objective requirement to change the droplet size, but this requirement can only be realized by using a more flexible single-package droplet, and it is difficult to use multiple packages. Droplets serve as microreactors while meeting the above-mentioned requirements for adjustable droplet size in multiple wrapping

Method used

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  • Enteric microorganism detection system based on micro-droplets
  • Enteric microorganism detection system based on micro-droplets
  • Enteric microorganism detection system based on micro-droplets

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Such as Figure 4 , 6 As shown, a micro-droplet-based intestinal microorganism detection system is provided, the detection system includes a reaction chip and a droplet preparation chip, and the two are fluidly connected through a connecting channel; the reaction chip includes a droplet distribution channel and micro-reaction chamber array; the droplet preparation chip is used to prepare double-wrapped droplets, including a cover sheet layer and a microchannel layer; a non-through microchannel structure is formed on the upper surface of the microchannel layer, and the The cover sheet layer covers the upper surface of the microchannel layer, and one side thereof is provided with four through holes in fluid communication with the microchannel layer; the microchannel structure includes an inner core fluid channel 1, a first continuous phase channel 2. Single-envelope droplet channel 3, second continuous phase channel 5, and double-envelope droplet channel 6; the core flui...

Embodiment 2

[0054] Such as Figure 5 As shown, the difference from Example 1 is that the connecting channel has a branch structure, and the branch structures are respectively connected to the reaction chips of the reaction chambers of different sizes, and the entrance of each branch structure is respectively provided with a microvalve for To selectively connect the branch structure; prepare double-wrapped droplets of different sizes by adjusting the operating parameters of the droplet preparation chip, and control the corresponding connection with the size of the double-wrapped droplet through the control of the microvalve Corresponding reaction chip.

Embodiment 3

[0056] Such as Image 6 As shown, a micro-droplet-based intestinal microorganism detection system is provided, which has a main structure similar to that of the micro-droplet preparation chip described in Example 1-2, the difference is that each of the single-wrapped droplet channels 3 The regulating channels 7 on the side are all composed of four sub-channels 71 arranged side by side, and the distance between any two adjacent dry sub-channels 71 is 0.5-3 times the width of the sub-channels 71 themselves.

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Abstract

The invention relates to an enteric microorganism detection system based on micro-droplets. The enteric microorganism detection system comprises a reaction chip and a droplet preparation chip which communicate with each other, and the droplet preparation chip is provided with an adjusting channel so that the size of multiple wrapped droplets can be accurately controlled and adjusted; according tothe change of droplet preparation demands, the multiple wrapped droplets with different sizes can be obtained through the change of power parameters of the adjusting channel; and meanwhile, adjustmentis not limited to the overall size of the multiple wrapped droplets, and the core size, the wrapping layer thickness and the like of the multiple wrapped droplet can all be independently adjusted. According to the droplet preparation chip of the enteric microorganism detection system, homogenized double emulsion droplets of different specifications can be allowed to be prepared on the same chip,universality within a certain droplet size range is achieved, the droplet preparation chip can serve as an independent droplet preparation unit to be combined with droplet use units of various specifications, and thus the detection cost is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a micro-droplet-based intestinal microorganism detection system. Background technique [0002] Intestinal microbes refer to the general term of microbial communities living in the human intestinal tract, including bacteria, archaea, and single-celled eukaryotes, which together with the intestinal environment constitute a huge and complex ecosystem. The human gut is a nutrient-rich microenvironment, carrying up to 100 trillion bacteria, and the number of genes in human gut microbes is 5 million, which is 150 times the number of human genes. Gut microbes are related to metabolic diseases, cardiovascular diseases, digestive system diseases, cancer, immune system diseases and central nervous system diseases. The research on their structure, function and pathogenic mechanism is beneficial to the treatment of diseases. and the development of new therapeutic modalities....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12Q1/6851
CPCC12M23/16C12M33/04C12Q1/6851C12Q2531/113C12Q2563/159
Inventor 徐迹秦颖李嵩山徐敏刘文兰徐勇黄建林
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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