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Method for biosynthesizing nano-silver bacteriostatic agent using Tibetan medicine willow tea extract

A technology for biosynthesis and silver bacteriostatic agent, applied in the field of biosynthesis of nano silver bacteriostatic agent, can solve the problems of difficult extraction method, difficult separation and utilization, waste of plant resources, medical resources and economic losses, etc. , Wide antibacterial spectrum, enhance the effect of antibacterial effect

Active Publication Date: 2021-06-01
NORTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Tibetan medicine willow tea is rich in antioxidant substances and antibacterial substances. However, due to the difficulty of its extraction method, it is difficult to separate and utilize it, so that it cannot exert its powerful antioxidative and antibacterial pharmacological effects, resulting in huge plant resources, medical resources and Economic loss and waste, the development and utilization of willow tea nano-silver will have great significance and economic value for the development and utilization of willow tea, a unique plant resource of the nation

Method used

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  • Method for biosynthesizing nano-silver bacteriostatic agent using Tibetan medicine willow tea extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) material preparation: collect Tibetan medicine willow tea leaves, clean and dry to constant weight to obtain dry willow tea leaves;

[0027] (2) Leaves of Tibetan medicine willow tea are placed under natural conditions to dry in the shade, dried in a blast drying oven at 45°C, crushed and passed through a 40-mesh sieve to obtain Tibetan medicine willow tea leaf powder, and 200g of Tibetan medicine willow tea leaf powder and 70 grams of Tibetan medicine willow tea leaf powder are accurately weighed. % ethanol was mixed at room temperature, heated and refluxed at 100°C for 30 min and repeated 3 times, the three extracts were combined, and vacuum evaporated at 35°C to obtain Tibetan medicine willow tea extract, which was diluted to 100 mg / mL with 70% ethanol;

[0028] (3) Preparation of Tibetan medicine willow tea nano-silver bacteriostatic agent: take Tibetan medicine willow tea extract and AgNO 3 Mix the solution, the mixing ratio is 30:1 according to the mass ratio ...

Embodiment 2

[0036] (1) material preparation: collect Tibetan medicine willow tea leaves, clean and dry to constant weight to obtain dry willow tea leaves;

[0037] (2) Preparation of Tibetan medicine willow tea extract: dry in a blast drying oven at 45°C, pulverize and pass through a 40-mesh sieve to obtain Tibetan medicine willow tea leaf powder, accurately weigh 200 g of Tibetan medicine willow tea leaf powder and 70% ethanol at room temperature Mixing, heating and refluxing at 100 °C for 30 min, repeated 3 times, combining the three extracts, vacuum evaporation at 35 °C to obtain Tibetan medicine willow tea extract, and diluting it to 100 mg / mL with 70% ethanol;

[0038] (3) Refinement and purification: the bacteriostatic agent obtained in step (2), bentonite, and diatomaceous earth are mixed and added to a three-necked flask, heated to 100° C., refluxed for 80 min, and the filtrate and filter residue after the reflux is finished, remove the filter residue, and recover the filtrate, Re...

Embodiment 3

[0044] This bacteriostatic agent is a single nano-AgNO 3 Solution bacteriostatic agent.

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Abstract

The invention provides a method for biosynthesizing a nano-silver bacteriostatic agent by using the extract of Tibetan medicine willow tea, which is prepared by (1) preparing materials; (2) preparing from the extract of Tibetan medicine willow tea; (3) antibacterial with nano-silver of Tibetan medicine willow tea (4) refining and purifying; (5) macroporous resin filtration. and other steps, using the rich antioxidant substances extracted from willow tea as a reducing agent, a one-step reduction method is used to prepare nano-silver antibacterial materials, and the antibacterial effect of willow tea itself can be used to synergistically improve the antibacterial effect, and the antibacterial effect is excellent.

Description

technical field [0001] The invention relates to a method for biosynthesizing nano-silver bacteriostatic agent by utilizing Tibetan medicine willow tea extract, and belongs to the field of natural product extraction. Background technique [0002] Silver is a kind of metal inorganic fungicides with a long history of application. Silver nanoparticles (AgNPs) are elements with nano-sized metal silver particles. The particle size of nano-silver is mostly around 25nm. Dozens of pathogenic microorganisms such as chlamydia have strong inhibitory and killing effects, and will not produce drug resistance. The antibacterial mechanism of nano-silver is: silver element or silver ion has a positive charge, which can combine with negatively charged microbial cell membrane, change the permeability of the membrane, and combine with bacterial protein and nucleic acid, destroy the ability of microorganisms to divide and proliferate, and finally lead to its death. It is the most widely studie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/34A01N59/16A01P1/00A01P3/00A61K36/73A61K33/38A61P31/04A61P31/10
CPCA01N59/16A01N65/00A01N65/34A61K33/38A61K36/73A61P31/04A61P31/10A61K2300/00Y02A50/30
Inventor 郭鹏辉边缘臧荣鑫曹忻徐红伟杨具田高丹丹韦体赫雪吴登宇
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
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