Primer and kit for detecting three common pathogen infection in dogs and application of kit

Abstract

The present invention discloses a primer and a kit for detecting three common canine pathogen infections in dogs, and an application of the kit. The primer comprises three primer sets for canine picoronavis (CPV), canine coronavirus (CCV) and canine distemper virus (CDV), each primer set consists of 6 primers of a F3 primer, a B3 primer, a FIP primer, a BIP primer, a LF primer, and a LB primer, and sequences vary depending on the targeted pathogens. The detection kit comprises three detection reagents of CPV, CCV, and CDV, and also comprise a negative control, a pretreatment solution A and a pretreatment solution B. A use method of the kit is as follows: dipping patient dog samples (for detection of CPV and CCV, taking stool or anus swabs and for detection of CDV, taking eye or nose secretion), putting the samples into each detection reagent after treatment, conducting reaction at constant temperature of 65 DEG C, and determining negative and positive results according to color changesafter completion. Based on a LAMP technology, pathogen gene fragments are specifically amplified, sampling and detection can be completed within 60 minutes, and an easiest operation method is used torealize high-precision detection results.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a primer, a kit and an application for detecting infection by three common pathogens in dogs. Background technique [0002] At present, pet pathogen detection mainly includes three methods: colloidal gold test paper, PCR test, and third-party testing services. Colloidal gold test paper is the most commonly used technology. Colloidal gold test paper technology developed earlier and is the easiest to operate, so it currently has the highest market share, accounting for about 70% of the pathogen testing market, and the representative companies are industry giants IDEXX and Lint Pharmaceuticals. However, due to the low accuracy of this technology, the positive coincidence rate is only about 60%, and the accuracy has gradually failed to meet the market demand, and the types of pathogens that can be tested are not perfect. For example, the most common three common pathogenic infectio...

AI Technical Summary

Problems solved by technology

However, due to the low accuracy of this technology, the positive coincidence rate is only about 60%, and the accuracy has gradually failed to meet the market demand, and the types of pathogens that can be tested are not perfect. For example, the most common three common pathogenic infections in dogs cannot be detected, etc. Therefore, its market share is shrinking year by year

[0003] PCR testing has entered the pet medical market from the human medical market in the past two years. Its biggest advantage is high accuracy, but the cost of its equipment is also very high. The price of a single instrument is more than 50,000, and the operation is also very complicated. , it is difficult for ordinary veterinarians to operate

Moreover, the installation of its equipment requires a self-built experimental space, and it is necessary to ensure that the experimental environment is well ventilated, otherwise false positives are easy to occur, and most pet hospitals do not have the installation conditions

Due to the above reasons, the promotion of in-hospital testing of the existing PCR testing technology is not good, and only a few central hospitals of leading chain pet hospital companies are equipped with it.

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