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Spirulina extract feed premix for penaeus vannamei

A technology of spirulina extraction and feed premix, which can be applied in animal feed, application, additional food elements, etc., can solve the problems of weakening the resistance of Vibrio parahaemolyticus, increasing the risk of vibrosis in shrimp, and decreasing immunity. To achieve the effect of improving immunity, improving anti-stress ability, inhibiting growth and replication

Pending Publication Date: 2020-03-06
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After Penaeus vannamei is subjected to different levels of ammonia nitrogen stress, the activities of phenoloxidase (PO), catalase (CAT) and superoxide dismutase (SOD) in the body are significantly reduced, which will cause the decline of shrimp immunity. , and weaken its resistance to Vibrio parahaemolyticus, increasing the risk of vibriosis in shrimp

Method used

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  • Spirulina extract feed premix for penaeus vannamei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 The preparation of the spirulina extract feed premix for Penaeus vannamei

[0017] Table 1 Feed premix formula for Penaeus vannamei with Spirulina extract

[0018]

[0019]

[0020] Refer to Table 1 to prepare the immune enhancers of formulas 1 to 5, weigh spirulina extract, water-soluble silicon dioxide (Shanghai Kaiyin Chemical Co., Ltd., 10kg / bag, A200) according to the formula, mix well, add appropriate ethanol to dissolve, and dry Dry, crush, pass through a No. 5 sieve, pack and seal for later use.

[0021] Among them, the spirulina extract is extracted in the following way:

[0022] Crush the spirulina (50 mesh), add distilled water according to the mass volume ratio of 1:15, add papain according to 5% of the mass of the dry powder of spirulina, mix well, and after 1 hour in water bath at 52℃~60℃, then in water bath at 100℃ for 0.5h~1.5 h, then 2-4 hours in a water bath at 80°C; filter through 8 layers of gauze (200 mesh), concentrate the supe...

Embodiment 2

[0023] Example 2 Study on Immunomodulatory Effect of Spirulina Extract Feed Premix on Penaeus vannamei

[0024] 1 Materials and methods

[0025] 1.1 Test drugs

[0026] The spirulina extract feed premix was prepared by the Pharmacology Laboratory of the College of Animal Science and Technology of Guangxi University according to the aforementioned Example 1. After the basic feed was pulverized, different doses of dry powder of spirulina extract were added according to the dose ratio of the test groups to make a granular feed premix, which was set aside.

[0027] Astragalus polysaccharide was donated by Beijing Shengtaier Biotechnology Co., Ltd. After the basic feed is crushed, astragalus polysaccharide is added according to the dosage ratio to make a granular feed premix, which is set aside.

[0028] 1.2 Test animals

[0029] The vannamei used in this experiment was provided by the Qinzhou Shrimp Breeding Base in Guangxi, with an average body length of 8.00±1.00cm and good ...

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Abstract

The invention discloses a spirulina extract feed premix for penaeus vannamei. The premix is prepared by mixing spirulina water extraction and alcohol precipitation extracts and auxiliary materials. Tests prove that after penaeus vannamei is fed with the feed premix containing the spirulina extract, the immunity and the anti-stress capability of penaeus vannamei organisms can be effectively improved. In addition, the product is derived from pure natural medicines, and has the advantages of low residue, low drug resistance, no toxic or side effect, good action effect and the like.

Description

technical field [0001] The invention belongs to the technical field of aquaculture feed, in particular to a spirulina extract feed premix for Penaeus vannamei. Background technique [0002] Penaeus vannamei is the fine variety with the highest single yield among the three major cultured prawns in the world, and it is also one of the most important economic shrimps in the world. Its meat is delicious, nutritious, and has high economic value. Since the 1990s, Penaeus vannamei has gradually become the most important shrimp cultured species in China, and its cultured volume accounts for more than 70% of the shrimps cultured in my country. [0003] In the actual production process, due to factors such as high breeding density and unreasonable feeding, it is very easy to cause the concentration of ammonia nitrogen in the water body to increase. Ammonia nitrogen is one of the most important hazard factors in aquaculture water, with NH 3 and NH 4 + Two forms exist, where NH 3 i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K20/00
CPCA23K50/80A23K20/00
Inventor 胡庭俊朱萌谢小东韦英益施君
Owner GUANGXI UNIV
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