A construction method and application of plasmids for interfering pig CRTC3 gene expression and adenoviruses
A technology of CRTC3 and construction method, applied in the field of biology and modern agriculture, can solve the problems of unknown regulation mechanism of subcutaneous fat and intramuscular fat difference, and achieve the effect of simple construction method
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[0061] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:
[0062] (1) Primer design for targeted interference with porcine CRTC3 gene expression
[0063] 1. According to the porcine CRTC3 sequence (Accession: MF964215.1) on NCBI and the carrier information, design 3 pairs of interference primers sh1~3, respectively:
[0064] sh1-F:5-GATCCGGAAGTTCAGCGAGAAGATTGTTCAAGAGACAATCTTCTCGCTGAACTTCCTTTTTTA-3
[0065] sh1-R:5-AGCTTAAAAAAGGAAGTTCAGCGAGAAGATTGTCTCTTGAACAATCTTCTCGCTGAACTTCCG-3
[0066] sh2-F:5-GATCCGGCTAACATCTGCACTCAACATTCAAGAGATGTTGAGTGCAGATGTTAGCCTTTTTTTA-3
[0067] sh2-R:5-AGCTTAAAAAAGGCTAACATCTGCACTCAACATCTCTTGAATGTTGAGTGCAGATGTTAGCCG-3
[0068] sh3-F:5-GATCCGCTCAATAAGACAGCGCTTTCTTCAAGAGAGAAAGCGCTGTCTTATTGAGCTTTTTTA-3
[0069] sh3-R:5-AGCTTAAAAAAGCTCAATAAGACAGCGCTTTCTCTCTTGAAGAAAGCGCTGTCTTATTGAGCG-3
[0070] 2. Three pairs of primers are annealed respectivel...
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