Metal-free coupling method, conjugate and its application

Abstract

The invention provides a metal-free coupling method, a conjugate and its application. The coupling method includes: coupling an active alkyne compound represented by the following formula I with a target having an alkyne-reactive functional group under metal-free conditions, wherein R and n are as defined herein. The coupling method of the present invention is fast and efficient and can be carriedout without heating and without the need for metal catalysts such as copper; therefore, the method has wide applicability.

Description

technical field [0001] The present invention relates to the field of biochemistry, in particular to a method for metal-free chemical conjugation, the resulting conjugates and applications thereof, such as applications for detection of target analytes such as microorganisms (for example, rapid staining of bacteria) and Preparation of fluorescent probes, kits, etc. Background technique [0002] In the field of biomedicine, it is very important to explore the structure of biomolecules and trace the internal mechanisms and processes of their functions, but it is very challenging to use technical means to "visualize" these mechanisms and processes. At present, most biomolecules and biomaterials do not have fluorescence that can be used for effective observation. Usually, coupled fluorescent molecules or other imaging units are used to realize the visual tracking of biological target molecules. Many biomolecules or biomedical materials need to be labeled and modified, such as pol...

AI Technical Summary

Problems solved by technology

For example, protein is an important structural and functional component in living organisms. Its structure and function are relatively complex, and its functional modification is difficult, which limits its biomedical application.

In addition, the fluorescent labeling of living organisms has always been a difficult problem. Traditionally, genetic methods are used, but the operation is complicated and the risk of uncertainty is high.

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