Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for determination of GP73 based on latex-enhanced immunoturbidimetry and production and application methods of kit

An immunoturbidimetric and latex-enhanced technology, which is applied in the fields of genetic engineering and immunological determination and analysis, can solve the problems of rapid outpatient detection, time-consuming, and complicated operation.

Pending Publication Date: 2020-03-06
ANHUI DAQIAN BIO ENG LIMITED
View PDF12 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzyme-linked immunosorbent assay method for GP73 detection can accurately detect the concentration of GP73 in the sample, but its operation is relatively complicated and time-consuming, which cannot meet the needs of rapid detection in outpatient or emergency departments

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for determination of GP73 based on latex-enhanced immunoturbidimetry and production and application methods of kit
  • Kit for determination of GP73 based on latex-enhanced immunoturbidimetry and production and application methods of kit
  • Kit for determination of GP73 based on latex-enhanced immunoturbidimetry and production and application methods of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] A kit for measuring GP73 based on the latex-enhanced immunoturbidimetric method of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0091] Reagent R1:

[0092]

[0093] Its solvent is purified water.

[0094] Reagent R2:

[0095]

[0096] Its solvent is purified water.

[0097] In addition, GP73 calibrator is also included, and the included ingredients and corresponding contents are:

[0098]

[0099] Its solvent is purified water.

[0100] Further, the rGP73 in the GP73 calibrator is recombinant human GP73 protein (obtained through purchasing channels).

[0101] Further, the method for obtaining cross-linked latex microspheres of anti-human rGP73 monoclonal antibody 1 in reagent R2 is: using polystyrene latex microspheres with a diameter of 80 nm, and chemically cross-linking anti-human rGP73 monoclonal antibody 1 Cross-linked to polys...

Embodiment 2

[0104] A kit for measuring GP73 based on the latex-enhanced immunoturbidimetric method of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0105] Reagent R1:

[0106]

[0107]

[0108] Its solvent is purified water.

[0109] Reagent R2:

[0110]

[0111] Its solvent is purified water.

[0112] In addition, GP73 calibrator is also included, and the included ingredients and corresponding contents are:

[0113]

[0114] Its solvent is purified water.

[0115] Further, the rGP73 in the GP73 calibrator is recombinant human GP73 protein (obtained through purchasing channels).

[0116] Further, the method for obtaining cross-linked latex microspheres of anti-human rGP73 monoclonal antibody 1 in reagent R2 is: using polystyrene latex microspheres with a diameter of 120 nm, and chemically cross-linking the anti-human rGP73 monoclonal antibody 1 Cros...

Embodiment 3

[0119] A kit for measuring GP73 based on the latex-enhanced immunoturbidimetric method of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0120] Reagent R1:

[0121]

[0122] Its solvent is purified water.

[0123] Reagent R2:

[0124]

[0125] Its solvent is purified water.

[0126] In addition, GP73 calibrator is also included, and the included ingredients and corresponding contents are:

[0127]

[0128] Its solvent is purified water.

[0129] Further, the rGP73 in the GP73 calibrator is recombinant human GP73 protein (obtained through purchasing channels).

[0130] Further, the method for obtaining cross-linked latex microspheres of anti-human rGP73 monoclonal antibody 1 in reagent R2 is: using polystyrene latex microspheres with a diameter of 100 nm, and chemically cross-linking anti-human rGP73 monoclonal antibody 1 Cross-linked to poly...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a kit for the determination of GP73 based on latex-enhanced immunoturbidimetry. The kit includes reagents R1 and R2. The reagent R1 includes PBS buffer solution, PEG6000, BSA, NaCl, Tween20, NaN3 and EDTA, and the reagent R2 includes PBS buffer solution, BSA, NaCl, latex microspheres crosslinked with anti-human rGP73 monoclonal antibody 1, latex microspheres crosslinked withanti-human rGP73 monoclonal antibody 2, NaN3 and EDTA. The invention also provides a method for the production and application of the kit for the determination of GP73 based on latex-enhanced immunoturbidimetry. The kit can be used on a full-automatic biochemical analyzer, and has the advantages of simple operation, low cost, high automation and saving detection time; and moreover, under the condition of high stability and high precision, the kit has higher sensitivity and specificity compared with other products.

Description

technical field [0001] The invention relates to the fields of genetic engineering and immunological assay and analysis, in particular to a kit for measuring GP73 based on a latex-enhanced immune turbidimetric method and a preparation method thereof. Background technique [0002] Golgi glycoprotein-73, also known as type II Golgi transmembrane protein, is called GP73 because its relative molecular mass is 7.3×104 in SDS-PAGE. Modern studies have shown that GP73 is highly expressed in various tumor tissues such as liver cancer, cholangiocarcinoma, prostate cancer, seminoma, and lung adenocarcinoma. Express. Recent studies have shown that GP73 is abnormally expressed in many diseases, but GP73 is closely related to liver diseases, especially liver cancer. It is expected to become a serological marker for early diagnosis of liver cancer, and its sensitivity is better than that of AFP. Serum GP73 level can be used as an index for evaluating the prognosis of liver diseases, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/577G01N33/543G01N33/531
CPCG01N33/68G01N33/577G01N33/54313G01N33/531G01N2333/47
Inventor 芮双印高耀辉
Owner ANHUI DAQIAN BIO ENG LIMITED
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com