Kit for determination of GP73 based on latex-enhanced immunoturbidimetry and production and application methods of kit

An immunoturbidimetric and latex-enhanced technology, which is applied in the fields of genetic engineering and immunological determination and analysis, can solve the problems of rapid outpatient detection, time-consuming, and complicated operation.

Pending Publication Date: 2020-03-06
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzyme-linked immunosorbent assay method for GP73 detection can accurately detect the concentration of GP73 in the sample, but it

Method used

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  • Kit for determination of GP73 based on latex-enhanced immunoturbidimetry and production and application methods of kit
  • Kit for determination of GP73 based on latex-enhanced immunoturbidimetry and production and application methods of kit
  • Kit for determination of GP73 based on latex-enhanced immunoturbidimetry and production and application methods of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] A kit for measuring GP73 based on the latex-enhanced immunoturbidimetric method of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0091] Reagent R1:

[0092]

[0093] Its solvent is purified water.

[0094] Reagent R2:

[0095]

[0096] Its solvent is purified water.

[0097] In addition, GP73 calibrator is also included, and the included ingredients and corresponding contents are:

[0098]

[0099] Its solvent is purified water.

[0100] Further, the rGP73 in the GP73 calibrator is recombinant human GP73 protein (obtained through purchasing channels).

[0101] Further, the method for obtaining cross-linked latex microspheres of anti-human rGP73 monoclonal antibody 1 in reagent R2 is: using polystyrene latex microspheres with a diameter of 80 nm, and chemically cross-linking anti-human rGP73 monoclonal antibody 1 Cross-linked to polys...

Embodiment 2

[0104] A kit for measuring GP73 based on the latex-enhanced immunoturbidimetric method of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0105] Reagent R1:

[0106]

[0107]

[0108] Its solvent is purified water.

[0109] Reagent R2:

[0110]

[0111] Its solvent is purified water.

[0112] In addition, GP73 calibrator is also included, and the included ingredients and corresponding contents are:

[0113]

[0114] Its solvent is purified water.

[0115] Further, the rGP73 in the GP73 calibrator is recombinant human GP73 protein (obtained through purchasing channels).

[0116] Further, the method for obtaining cross-linked latex microspheres of anti-human rGP73 monoclonal antibody 1 in reagent R2 is: using polystyrene latex microspheres with a diameter of 120 nm, and chemically cross-linking the anti-human rGP73 monoclonal antibody 1 Cros...

Embodiment 3

[0119] A kit for measuring GP73 based on the latex-enhanced immunoturbidimetric method of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0120] Reagent R1:

[0121]

[0122] Its solvent is purified water.

[0123] Reagent R2:

[0124]

[0125] Its solvent is purified water.

[0126] In addition, GP73 calibrator is also included, and the included ingredients and corresponding contents are:

[0127]

[0128] Its solvent is purified water.

[0129] Further, the rGP73 in the GP73 calibrator is recombinant human GP73 protein (obtained through purchasing channels).

[0130] Further, the method for obtaining cross-linked latex microspheres of anti-human rGP73 monoclonal antibody 1 in reagent R2 is: using polystyrene latex microspheres with a diameter of 100 nm, and chemically cross-linking anti-human rGP73 monoclonal antibody 1 Cross-linked to poly...

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Abstract

The invention provides a kit for the determination of GP73 based on latex-enhanced immunoturbidimetry. The kit includes reagents R1 and R2. The reagent R1 includes PBS buffer solution, PEG6000, BSA, NaCl, Tween20, NaN3 and EDTA, and the reagent R2 includes PBS buffer solution, BSA, NaCl, latex microspheres crosslinked with anti-human rGP73 monoclonal antibody 1, latex microspheres crosslinked withanti-human rGP73 monoclonal antibody 2, NaN3 and EDTA. The invention also provides a method for the production and application of the kit for the determination of GP73 based on latex-enhanced immunoturbidimetry. The kit can be used on a full-automatic biochemical analyzer, and has the advantages of simple operation, low cost, high automation and saving detection time; and moreover, under the condition of high stability and high precision, the kit has higher sensitivity and specificity compared with other products.

Description

technical field [0001] The invention relates to the fields of genetic engineering and immunological assay and analysis, in particular to a kit for measuring GP73 based on a latex-enhanced immune turbidimetric method and a preparation method thereof. Background technique [0002] Golgi glycoprotein-73, also known as type II Golgi transmembrane protein, is called GP73 because its relative molecular mass is 7.3×104 in SDS-PAGE. Modern studies have shown that GP73 is highly expressed in various tumor tissues such as liver cancer, cholangiocarcinoma, prostate cancer, seminoma, and lung adenocarcinoma. Express. Recent studies have shown that GP73 is abnormally expressed in many diseases, but GP73 is closely related to liver diseases, especially liver cancer. It is expected to become a serological marker for early diagnosis of liver cancer, and its sensitivity is better than that of AFP. Serum GP73 level can be used as an index for evaluating the prognosis of liver diseases, and ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/543G01N33/531
CPCG01N33/68G01N33/577G01N33/54313G01N33/531G01N2333/47
Inventor 芮双印高耀辉
Owner ANHUI DAQIAN BIO ENG LIMITED
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