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Anti-Mullerhormone latex enhanced turbidimetry detection kit, and preparation and application method thereof

A detection kit and latex-enhanced technology, applied in the field of genetic engineering technology and immunological assay and analysis, can solve the problems of low specificity and high detection cost

Active Publication Date: 2019-07-05
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, in view of the problems of low specificity and high detection cost of existing automated detection kits, there is an urgent need for an anti-Müllerian hormone latex-enhanced turbidimetric detection kit based on a polyclonal antibody cross-linked substrate, which can maintain sensitivity while maintaining sensitivity. , which can further improve the specificity of detection and further reduce the cost of detection

Method used

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  • Anti-Mullerhormone latex enhanced turbidimetry detection kit, and preparation and application method thereof
  • Anti-Mullerhormone latex enhanced turbidimetry detection kit, and preparation and application method thereof
  • Anti-Mullerhormone latex enhanced turbidimetry detection kit, and preparation and application method thereof

Examples

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Effect test

Embodiment 1

[0101] An anti-Müllerian hormone latex-enhanced turbidimetric detection kit of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0102] Reagent R1:

[0103]

[0104] The solvent is purified water;

[0105] Reagent R2:

[0106]

[0107] Its solvent is purified water.

[0108] In addition, AMH calibrators are also included, and the included ingredients and corresponding contents are as follows:

[0109]

[0110] Its solvent is purified water.

[0111] Further, the rAMH in the AMH calibrator is specifically recombinant human AMH protein (see Example 4 for the preparation method).

[0112] Further, the method for obtaining the latex microspheres of the cross-linked goat anti-human AMH polyclonal antibody in reagent R2 is: using polystyrene latex microspheres with a diameter of 30-60 nm, and covalently coupling the goat anti-human AMH polyclonal anti...

Embodiment 2

[0114] An anti-Müllerian hormone latex-enhanced turbidimetric detection kit of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0115] Reagent R1:

[0116]

[0117]

[0118] The solvent is purified water;

[0119] Reagent R2:

[0120]

[0121] Its solvent is purified water.

[0122] In addition, AMH calibrators are also included, and the included ingredients and corresponding contents are as follows:

[0123]

[0124] Its solvent is purified water.

[0125] Further, the rAMH in the AMH calibrator is specifically recombinant human AMH protein (see Example 4 for the preparation method).

[0126] Further, the method for obtaining the latex microspheres of the cross-linked goat anti-human AMH polyclonal antibody in reagent R2 is: using polystyrene latex microspheres with a diameter of 30-60 nm, and covalently coupling the goat anti-human AMH pol...

Embodiment 3

[0128] An anti-Müllerian hormone latex-enhanced turbidimetric detection kit of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0129] Reagent R1:

[0130]

[0131] The solvent is purified water;

[0132] Reagent R2:

[0133]

[0134] Its solvent is purified water.

[0135] In addition, AMH calibrators are also included, and the included ingredients and corresponding contents are as follows:

[0136]

[0137]

[0138] Its solvent is purified water.

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Abstract

The invention provides an anti-Mullerhormone latex enhanced turbidimetry detection kit. The kit includes a reagent R1 and a reagent R2; the reagent R1 includes HEPES, KH2PO4, PEG6000, BSA, etc.; and the reagent R2 includes HEPES, KH2PO4, PEG6000, latex microspheres crosslinked with goat anti-human AMH polyclonal antibodies, etc. A preparation and application method for the kit is also provided. The kit firstly utilizes a latex enhanced immunity transmission turbidity method to determine anti-Mullerhormone, and can be used on a fully automatic biochemical analyzer, so that the kit is low in cost and high in automation, and therefore, detection time can be greatly saved; and in the case of high stability and precision, the kit has higher sensitivity than ELISA products and has better specificity than chemiluminescent products, so that the clinical application values of anti-Mullerhormone detection can be greatly enhanced.

Description

technical field [0001] The invention relates to the technical field of genetic engineering and the field of immunological determination and analysis, in particular to an anti-Müllerian hormone latex enhanced turbidimetric detection kit and a preparation method thereof. Background technique [0002] Anti-Mullerian hormone (Anti-Mullerhormone, AMH) is a member of the transforming growth factor β (TGF-β) superfamily and one of the markers of gonadal function. It can induce the degeneration of Mullerian ducts and plays an important role in the development of gonad organs. Male AMH is mainly produced by immature Sertoli cells of the testis, which is formed in the embryonic period and lasts throughout life; female AMH is mainly produced by granulosa cells of ovarian preantral follicles and small antral follicles, and its level is lower than that of males; when female development reaches After puberty, AMH levels gradually decrease and are barely detectable by menopause. AMH is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/531G01N33/536G01N33/74C12N15/70C07K14/495C07K1/22C07K1/18C07K16/22C07K16/06
CPCG01N33/531G01N33/5306G01N33/53G01N33/536G01N33/74C12N15/70C07K14/495C07K16/22C07K16/06C07K16/065
Inventor 符修乐吴瑶瑶芮双印王俊薇陆大伟郭荣芳程虎支新梅高帆
Owner ANHUI DAQIAN BIO ENG LIMITED
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