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Anti-Mullerian hormone latex-enhanced turbidimetric detection kit and its preparation and use method

A detection kit and latex enhancement technology are applied in the field of genetic engineering technology and the field of immunological assay analysis, which can solve the problems of high detection cost and low specificity, and achieve the effects of high sensitivity, low cost and good specificity.

Active Publication Date: 2022-04-12
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, in view of the problems of low specificity and high detection cost of existing automated detection kits, there is an urgent need for an anti-Müllerian hormone latex-enhanced turbidimetric detection kit based on a polyclonal antibody cross-linked substrate, which can maintain sensitivity while maintaining sensitivity. , which can further improve the specificity of detection and further reduce the cost of detection

Method used

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  • Anti-Mullerian hormone latex-enhanced turbidimetric detection kit and its preparation and use method
  • Anti-Mullerian hormone latex-enhanced turbidimetric detection kit and its preparation and use method
  • Anti-Mullerian hormone latex-enhanced turbidimetric detection kit and its preparation and use method

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Effect test

Embodiment 1

[0101] An anti-Mullerian hormone latex-enhanced turbidimetric detection kit of the present embodiment includes two independent reagent R1 and reagent R2 two-liquid components, and the components and corresponding contents are as follows:

[0102] Reagent R1:

[0103]

[0104] Its solvent is purified water;

[0105] Reagent R2:

[0106]

[0107] Its solvent is purified water.

[0108] In addition, AMH calibrators are also included, including the following components and corresponding contents:

[0109]

[0110] Its solvent is purified water.

[0111] Further, rAMH in the AMH calibrator is specifically recombinant human AMH protein (see Example 4 for the preparation method).

[0112] Further, the method for obtaining latex microspheres cross-linked with goat anti-human AMH polyclonal antibody in reagent R2 is as follows: polystyrene latex microspheres with a diameter of 30-60 nm are used, and goat anti-human AMH polyclonal antibodies are covalently coupled by using ...

Embodiment 2

[0114] An anti-Mullerian hormone latex-enhanced turbidimetric detection kit of the present embodiment includes two independent reagent R1 and reagent R2 two-liquid components, and the components and corresponding contents are as follows:

[0115] Reagent R1:

[0116]

[0117]

[0118] Its solvent is purified water;

[0119] Reagent R2:

[0120]

[0121] Its solvent is purified water.

[0122] In addition, AMH calibrators are also included, including the following components and corresponding contents:

[0123]

[0124] Its solvent is purified water.

[0125] Further, rAMH in the AMH calibrator is specifically recombinant human AMH protein (see Example 4 for the preparation method).

[0126] Further, the method for obtaining latex microspheres cross-linked with goat anti-human AMH polyclonal antibody in reagent R2 is as follows: polystyrene latex microspheres with a diameter of 30-60 nm are used, and goat anti-human AMH polyclonal antibodies are covalently coupl...

Embodiment 3

[0128] An anti-Mullerian hormone latex-enhanced turbidimetric detection kit of the present embodiment includes two independent reagent R1 and reagent R2 two-liquid components, and the components and corresponding contents are as follows:

[0129] Reagent R1:

[0130]

[0131] Its solvent is purified water;

[0132] Reagent R2:

[0133]

[0134] Its solvent is purified water.

[0135] In addition, AMH calibrators are also included, including the following components and corresponding contents:

[0136]

[0137]

[0138] Its solvent is purified water.

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Abstract

The invention provides an anti-Mullerian hormone latex enhanced turbidimetric detection kit, including reagent R1 and reagent R2, reagent R1: HEPES, KH 2 PO 4 , PEG6000, BSA, etc.; Reagent R2: HEPES, KH 2 PO 4 , PEG6000, latex microspheres of cross-linked goat anti-human AMH polyclonal antibody, etc. The invention also provides a method for preparing and using an anti-Müllerian hormone latex-enhanced turbidimetric method detection kit. The present invention utilizes latex-enhanced immune transmission turbidimetry for the first time to measure anti-Müllerian hormone, which can be used on a fully automatic biochemical analyzer, with low cost and high automation, and can greatly save detection time; moreover, it has high stability and high precision Under certain circumstances, it has higher sensitivity than ELISA products and better specificity than chemiluminescence products, which greatly improves the clinical application value of anti-Mullerian hormone detection.

Description

technical field [0001] The invention relates to the technical field of genetic engineering and the field of immunological assay analysis, in particular to an anti-Mullerian hormone latex-enhanced turbidimetric detection kit and a preparation and use method thereof. Background technique [0002] Anti-Mullerhormone (AMH) is a member of the transforming growth factor β (TGF-β) superfamily and one of the markers of gonadal function. It can induce Mullerian duct regression and play an important role in the development of gonadal organs. Male AMH is mainly produced by immature Sertoli cells of the testis, formed during the embryonic period, and continues throughout life; female AMH is mainly produced by granulosa cells of preantral follicles and small antral follicles of the ovary, and its level is lower than that of males; After puberty, AMH levels gradually decrease and are barely detectable by menopause. AMH is a diglycan protein composed of two identical subunits through disu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/531G01N33/536G01N33/74C12N15/70C07K14/495C07K1/22C07K1/18C07K16/22C07K16/06
CPCG01N33/531G01N33/5306G01N33/53G01N33/536G01N33/74C12N15/70C07K14/495C07K16/22C07K16/06C07K16/065
Inventor 芮双印符修乐吴瑶瑶王俊薇陆大伟郭荣芳程虎支新梅高帆
Owner ANHUI DAQIAN BIO ENG LIMITED
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