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Detection kit for TIMP-I latex enhanced turbidimetry and preparation and using method thereof

A technology of TIMP-I and detection kit, which is applied in the field of biological detection and immunological determination and analysis, and can solve the problems of low degree of automation, poor repeatability and stability, etc.

Inactive Publication Date: 2020-02-07
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The main purpose of the present invention is to propose a serum tissue inhibitor of metalloproteinase I latex-enhanced turbidimetric detection kit and its preparation and use method prepared based on polyclonal antibody, aiming to solve the automation degree existing in the existing TIMP-I detection method bottom and poor repeatability and stability

Method used

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  • Detection kit for TIMP-I latex enhanced turbidimetry and preparation and using method thereof
  • Detection kit for TIMP-I latex enhanced turbidimetry and preparation and using method thereof
  • Detection kit for TIMP-I latex enhanced turbidimetry and preparation and using method thereof

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Embodiment 1

[0132] A TIMP-I latex-enhanced turbidimetric detection kit of the present embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0133] Reagent R1:

[0134]

[0135] Reagent R2:

[0136]

[0137] In addition, TIMP-I calibrator is also included, and the ingredients and corresponding contents are as follows:

[0138]

[0139]

[0140] Further, rTIMP-I in the TIMP-I calibrator is specifically recombinant human TIMP-I protein (see Example 4 for the preparation method).

[0141] Further, the method for obtaining latex microspheres of TIMP-I polyclonal antibody in reagent R2 is as follows: using polystyrene latex microspheres with a diameter of 100-300 nm, and chemically cross-linking the goat anti-human TIMP-I polyclonal antibody (see Example 4 for the preparation method) cross-linked to polystyrene latex microspheres.

Embodiment 2

[0143] A TIMP-I latex-enhanced turbidimetric detection kit of the present embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0144] Reagent R1:

[0145]

[0146] Reagent R2:

[0147]

[0148]

[0149] In addition, TIMP-I calibrator is also included, and the ingredients and corresponding contents are as follows:

[0150]

[0151] Further, rTIMP-I in the TIMP-I calibrator is specifically recombinant human TIMP-I protein (see Example 4 for the preparation method).

[0152] Further, the method for obtaining latex microspheres of TIMP-I polyclonal antibody in reagent R2 is as follows: using polystyrene latex microspheres with a diameter of 100-300 nm, and chemically cross-linking the goat anti-human TIMP-I polyclonal antibody (see Example 4 for the preparation method) cross-linked to polystyrene latex microspheres.

Embodiment 3

[0154] A TIMP-I latex-enhanced turbidimetric detection kit of the present embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0155] Reagent R1:

[0156]

[0157] Reagent R2:

[0158]

[0159]

[0160] In addition, TIMP-I calibrator is also included, and the ingredients and corresponding contents are as follows:

[0161]

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Abstract

The invention discloses a detection kit by TIMP-I latex enhanced turbidimetry, and the kit comprises two liquid components of a reagent R1 and a reagent R2 which are independent of each other, whereinthe reagent R1 includes Tris-HCl buffer, PEG6000, BSA, NaCl, MgCl2, NaN3 and EDTA; the reagent R2 includes Tris-HCl buffer, PEG6000, BSA, TIMP-I polyclonal antibody latex microspheres, TX-100, NaCl,MgCl2, NaN3 and EDTA. The invention also discloses a method for preparing and using the above-mentioned detection kit for TIMP-I latex enhanced turbidimetry. The kit provided by the invention has goodrepeatability and stability, high sensitivity and specificity, and can be used on a full-automatic biochemical analyzer with low cost, can save detection time, and facilitates clinical application.

Description

technical field [0001] The present invention relates to the fields of biological detection and immunological determination and analysis, in particular to a serum tissue metalloproteinase inhibitor I latex-enhanced turbidimetric detection kit prepared based on a polyclonal antibody and a preparation method thereof. Background technique [0002] TIMPs are the most important family of enzymes regulating the activity of extracellular MMPs, and are active proteins with many common biochemical and physiological properties. At present, four members are found, TIMP1-4. Among them, TIM P-1 can inhibit the activity of most MMPs, especially MMP-1, which is considered to play an important role in liver fibrosis. TIMP-1 was first isolated from rabbit skeletal muscle with a molecular weight of 28.5kD. The coding gene is located in the P11 region of the X chromosome and contains an open reading frame region. Its protein molecule contains 184 amino acids, forming three ring structures. It...

Claims

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Application Information

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IPC IPC(8): G01N33/573G01N33/543G01N33/531G01N21/82
CPCG01N33/573G01N33/54313G01N33/531G01N21/82G01N2333/96486
Inventor 芮双印李祥宇支新梅
Owner ANHUI DAQIAN BIO ENG LIMITED
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