Method for screening and identifying strains with high glucosinolate degradation capacity

Abstract

The invention discloses a method for screening and identifying strains with high glucosinolate degradation capacity. The method comprises the following steps: S1, extracting glucosinolate, determiningthe content of glucosinolate, and setting the concentration of a glucosinolate standard substance; S2, purifying the strain by using MRS and PDA as culture media, carrying out morphological observation, carrying out liquid culturing on a single bacterial colony, and then carrying out strain preservation and gram staining on the strain; S3, determining the glucosinolate degradation rate of the strain, carrying out first screening, carrying out tolerance experiment on the screened strain, and carrying out second screening; and S4, carrying out rapid molecular biological identification on the screened strain by using a 16S rDNA gene sequence analysis method.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation measurement of sinapinin, in particular to a strain screening and identification method with high sinapinin degradation ability. Background technique [0002] 1. Brief introduction of sinaprin [0003] Sinoglucosinolate, also known as glucosinolate, is a glucoside formed by combining aglycone containing a sulfhydryl group (-SH) with glucose, and mainly exists in cruciferous plants. Simple structure such as figure 1 , where R is various aryls and alkanes. Myrosinin is an anti-nutritional factor, and myrosinin can be degraded by myrosinase or heat to generate isothiocyanate. [0004] 2. Study on the characteristics of isothiocyanate, the degradation product of sinaprin [0005] Isothiocyanates are a family of compounds characterized by sulfur containing N=C=S functional groups. It is an important and unique class II plant product. Isothiocyanates are widely used in food, medicine...

AI Technical Summary

Problems solved by technology

[0010] 4. Traditional microbial identification methods

But studies have shown that there are many microorganisms that cannot be achieved using current culture techniques

Therefore the traditional method has its limitations, side illustration of the diversity of microorganisms

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