Dynamic cell culture method and culture device for simulating in vivo force-electric microenvironment
A technology of cell culture and microenvironment, which is applied in the field of dynamic cell culture method and culture device that simulates the force-electric microenvironment in vivo, and can solve the problem that the cell culture method and culture device cannot accurately simulate the complex force-electric microenvironment of cells, Achieve the effect of accurately simulating the internal environment
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Embodiment 1
[0048] The dynamic cell culture method that simulates the force-electric microenvironment in vivo is to plant cells on flexible substrates for culture in vitro, and apply cyclic dynamic loads and cyclic dynamic electric fields to the flexible substrates during the culture process to realize the simulation of human cardiac muscle A dynamic cell culture method for the force-electric microenvironment of the myocardium during exercise;
[0049] The application frequency of cyclic dynamic load and cyclic dynamic electric field is f, the value range of f is 0.5~2Hz, and the application direction of cyclic dynamic load is D F ,D F is the direction perpendicular to the flexible substrate, and the applied direction of the cyclic dynamic electric field is a direction parallel to the flexible substrate;
[0050] The start time and frequency of the cyclic dynamic load and the cyclic dynamic electric field are consistent;
[0051] In each cycle period, the load increases gradually with t...
Embodiment 2
[0056] The dynamic cell culture method that simulates the force-electric microenvironment in vivo is to plant cells on flexible substrates for cultivation in vitro, and apply cyclic dynamic loads and cyclic dynamic electric fields to the flexible substrates during the culture process to realize the simulation of human growth. A dynamic cell culture method for the mechano-electric microenvironment of cells at osteogenesis during the bone process;
[0057] The application frequency of cyclic dynamic load and cyclic dynamic electric field is f, the value range of f is 2-30Hz, and the application direction of cyclic dynamic load is D F ,D F is the direction perpendicular to the flexible substrate, and the applied direction of the cyclic dynamic electric field is a direction parallel to the flexible substrate;
[0058] The start time and frequency of the cyclic dynamic load and the cyclic dynamic electric field are consistent;
[0059] In each cycle period, the load increases gra...
Embodiment 3
[0064] The dynamic cell culture method that simulates the in vivo force-electric microenvironment is to plant cells on flexible substrates for culture in vitro, and apply cyclic dynamic loads and cyclic dynamic electric fields to the flexible substrates during the culture process to realize the simulation of human neurons. Dynamic cell culture method of cell force-electric microenvironment;
[0065] The applied frequency of cyclic dynamic load and cyclic dynamic electric field is f, the value range of f is 0.1~200Hz, and the applied direction of cyclic dynamic load is D F ,D F is the direction parallel to the flexible substrate, and the application direction of the cyclic dynamic electric field is parallel to the direction of the flexible substrate;
[0066] The start time and frequency of the cyclic dynamic load and the cyclic dynamic electric field are consistent;
[0067] In each cycle period, the load increases gradually with time first, and then maintains for a certain ...
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