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Applications of ILT4 in tumor diagnosis and treatment

A tumor and expression product technology, applied in the fields of biomedicine and molecular biology, can solve the problem of unclear direct regulation of tumor-infiltrating T cell fate and function

Active Publication Date: 2020-03-24
JINAN CENTER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the direct regulation of tumor-derived ILT4 on the fate and function of tumor-infiltrating T cells is still unclear.

Method used

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  • Applications of ILT4 in tumor diagnosis and treatment
  • Applications of ILT4 in tumor diagnosis and treatment
  • Applications of ILT4 in tumor diagnosis and treatment

Examples

Experimental program
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Embodiment 1

[0042] 1. Cell culture related experiments

[0043] 1.1 Cell culture

[0044] Human cell lines A549, H1299, H1650, MCF7, ZR751, M628, PC-3, 293T and mouse cell lines E0771, B16F0 were cultured with RPMI-1640 or DMEM containing 10% FBS and 1% penicillin and streptomycin; Mouse lung cancer cell line MLE12 was cultured in DMEM containing 2% fetal bovine serum, 0.005mg / ml insulin, ITS (1:100), 10nM hydrocortisone, 10nMβ-estradiol, 2mML-glutamine; Lung cancer cell line LL / 2 was cultured in high-glucose DMEM containing 10% FBS, 2% L-glutamine, and 1% penicillin-streptomycin; MCF10A was cultured in 2% fetal calf serum, 1 μg / ml hydrocortisone , 20ng / ml EGF and 1.2μg / ml insulin in DMEM; FSK4 was cultured in DMEM containing 2% FBS, 1% HEPES solution, 10μg / ml insulin and 5ng / ml EGF.

[0045] PBMC from healthy people were separated by Ficoll-Paque cell separation medium, human CD4 + and CD8 + T cells were sorted from PBMC using the corresponding magnetic bead cell sorting kit, and flo...

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Abstract

The invention provides applications of ILT4 in tumor diagnosis and treatment. Real-time PCR and flow cytometry are adopted to detect expression of ILT4 in human tumor cell strains and epithelial cellstrains, immunohistochemistry is adopted to detect expression of ILT4 in tissues of NSCLC and breast cancer patients, and correlation between ILT4 expression and clinical pathological features and overall survival (OS) of patients is statistically analyzed; the correlation between the expression of ILT4 in human NSCLC, breast cancer, prostate cancer and malignant melanoma tumor tissues and patientprognosis is analyzed; it is comprehensively demonstrated that high expression of ILT4 is a general characteristic of malignant tumors, and adverse prognosis of patients is predicted. Meanwhile, theILT4 in ILT4 low / high expression tumor cells is respectively regulated up / down by utilizing a gene transfection and knockout technology, and the induction effect of the ILT4 on T cell aging is definedthrough a co-culture system of the tumor cells and the T cells, so that a high practical application value is achieved.

Description

technical field [0001] The invention belongs to the technical fields of biomedicine and molecular biology, and specifically relates to the application of ILT4 in tumor diagnosis and treatment. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Immunoglobulin-like transcript 4 (ILT4), also known as lymphocyte immunoglobulin-like receptor B2 (LILRB2), monocyte-macrophage immunoglobulin-like receptor 10 (MIR10), or CD85d, belongs to the class of immunoglobulin-like family of transcripts (ILTs). This family includes 6 activating receptor members and 5 inhibitory receptor members, which are immune to arginine residues and intracellular regions of the transmembrane ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61P35/00
CPCA61K45/00A61P35/00C12Q1/6886C12Q2600/118C12Q2600/158G01N33/57415G01N33/57423G01N33/5743G01N33/57434G01N33/57484G01N2333/70503G01N2800/52
Inventor 孙玉萍高爱琴王婧男王淑云林文俐李娟刘帅
Owner JINAN CENTER HOSPITAL
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