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A target for tumor therapy and its application

A tumor and tumor cell technology, applied to the target of tumor therapy and its application field, can solve problems such as difficulty in proposing a treatment plan

Active Publication Date: 2020-12-11
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high heterogeneity of tumor cells, it is difficult to propose effective treatment options from the perspective of genetic diversity and epigenetics

Method used

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  • A target for tumor therapy and its application
  • A target for tumor therapy and its application
  • A target for tumor therapy and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Example 1. Tumor cells in various clinical samples express BTN3A2 and BTN3A3

[0094] 1. Isolation of tumor cells from clinical samples

[0095] (1) Preparation of digestive solution: 20 ml of 1640 medium (Hyclone Company, SH30809), 20 mg of collagenase IV and 1 mg of DNase I were mixed to obtain a digestive solution, which was filtered through a 0.45 μm filter membrane.

[0096] (2) Take fresh tumor tissues from clinical patients, cut them into pieces, put them into the digestive solution prepared in step (1) to obtain tumor digestive solution, and digest them at 37° C. for 40 minutes.

[0097] (3) Filter the tumor digestion solution with a 70 μm filter, and centrifuge at 250 g for 10 min.

[0098] (4) Wash the tumor cells in the tumor digestion solution with 1640 medium for 3 times, and obtain enriched tumor cell subpopulations by flow cytometry: the specific steps are as follows: the living cell population is passed through SSC-H / FSC-W and SSC-W / FSC-H removes adher...

Embodiment 2

[0117] Example 2, various tumor cell lines express BTN3A3

[0118] 1. Culture of tumor cell lines

[0119] 培养如下乳腺癌细胞系:MCF7(3111C0001CCC000013)、ZR75-1(3111C0001CCC000090)、BT474(3111C0001CCC000129)、T47D(3111C0001CCC000265)、MDA-MB-453(3111C0001CCC000016)、SKBR3(3111C0001CCC000085)、MDA-MB-468(3111C0001CCC000249 )、MDA-MB-436(3111C0001CCC000352);MDA-MB-231(3111C0001CCC000013);肝癌细胞系:BEL-7402(3131C0001000700010)、HepG2(3111C0001CCC000035)、HCC-LM3(3142C0001000000316)、HHCC(3111C0002000000069)、Hep3B( 3111C0001CCC000376)、QGY7701(3131C0001000700042)、SMCC7721(3111C0001CCC000087)、Huh7(3131C0001000700182);黑色素瘤细胞系:A875(3111C0001CCC000094)、A375(3131C0001000700004);胃癌细胞系:MKN28(3111C0001CCC000482)、NCI-N87(3111C0001CCC000481)、MGC -803 (311c0001CCC000227), SGC-7901 (3131C0001000700046); colon cancer cell line: LOVO (3111c0001ccc000164), SW480 (3142C0001000000064), LS174T (3111c0001CC000000248), DLD-1 (313131313) (3131313131313) (313131313131 (31313131313) (31313131313) The above cells were purchase...

Embodiment 3

[0122] Expression of embodiment 3, BTN3A2 and BTN3A3 on breast cancer cells

[0123] 1. qPCR detection of expression levels of BTN3A2 and BTN3A3 on breast cancer cell lines

[0124] 1. Extraction of RNA and reverse transcription of cDNA

[0125] Use the RNA extraction kit to extract the following breast cancer cells: MCF7, ZR75-1, BT474, T47D, SKBR3, MDA-MB-468, MDA-MB-231, MDA-MB-436 RNA; Methods cDNA was synthesized.

[0126] 2. qPCR detection of BTN3A3 expression level on breast cancer cell lines

[0127] Using the cDNA obtained in step 1 as a template, a real-time fluorescent quantitative nucleic acid amplification detection system (qPCR) was used to amplify BTN3A2, BTN3A3 and GAPDH, and the relative expression levels of BTN3A2 and BTN3A3 were analyzed by software. The above BTN3A2, BTN3A3 and GAPDH primers were purchased from Qiagen.

[0128] The result is as image 3 (a) shown. The results of qPCR detection showed that BTN3A2 and BTN3A3 were highly expressed in bre...

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Abstract

The invention discloses tumor treatment targets and applications thereof. According to the present invention, the experiment results prove that LSECtin, BTN3A2 and BNT3A3 promote tumor progression bypromoting the tumor cell stemness maintenance, wherein the promotion of the tumor progression specifically comprises: promoting the tumor cell sphere formation, expressing stemness transcription factors, and promoting the tumor progression in mouse tumor models, such that the inhibition on the interaction of LSECtin and BTN3A2 and the interaction of LSECtin and BNT3A3 can effectively retard the tumor progression, wherein the retarding of the tumor progression specifically comprises: reducing the tumor incidence, and retarding the tumor volume increase.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a tumor treatment target and its application. Background technique [0002] The global incidence of cancer has been on the rise since the late 1970s. At present, the treatment methods for breast cancer mainly include surgery, radiotherapy, chemotherapy, endocrine therapy, biological targeted therapy and Chinese medicine adjuvant therapy, etc. The fundamental problem that plagues cancer treatment is drug resistance and recurrence after cure. Studies have shown that the root cause of drug resistance and recurrence lies in the enhancement of tumor cell stemness. For example, triple-negative breast cancers characterized by estrogen receptor (ER), progesterone receptor (PR), epidermal growth factor receptor 2 (HER2) negative tend to be considered to have a higher tumor stemness property. There are also research results showing that most non-triple-negative breast cancer pati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61P35/00
CPCA61K45/00
Inventor 唐丽贺福初柳迪王兴
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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