A yeast y5 resistant to aquatic pathogens and producing lipase, its separation method and application, feed additive and its preparation method
A technology of feed additive and lipase, which is applied in the field of microorganisms, can solve the problems of drug residues and other problems, and achieve the effect of improving the harm of the human body
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Embodiment 1
[0041] Example 1: Isolation and Identification of Saccharomyces Y5
[0042] Be that the streptomycin of 50ml / L and the penicillin of 50ml / L are added in the YPD solid plate, then the 2089 meters deep in the southwest Indian Ocean (36.22°S, 52.86°E) collected by 5g Dayang 21 voyage The sediment sample was applied to the plate, and after 48 hours, a single colony was picked with a toothpick and cultured at 28°C to isolate the yeast.
[0043] After placing the yeast Y5 on a YPD plate at 28°C for 72 hours, the front and back of the colony are pink; the surface of the colony is full, round, shiny and neat, the color of the center of the colony is consistent with that of the edge, and the single colony is round and the size is 2mm left and right; surface, viscous, easy to provoke, such as figure 1 and figure 2 shown. Under the scanning electron microscope, the yeast is distributed singly or in clusters, the single cell is 3-5 microns, and it is round to oval, such as image 3 s...
Embodiment 2
[0057] Embodiment 2: the optimal preparation of yeast Y5 fermentation medium
[0058] Based on the above culture conditions, the optimal preparation of yeast Y5 fermentation medium was carried out. The specific experiments are as follows:
[0059] The present invention has selected a variety of carbon sources (sucrose, glucose, soluble starch, molasses), nitrogen sources (peptone, beef extract, cornmeal) and inorganic salts (MgSO 4 ,NaCl,KH 2 PO 4 ), under the same culture conditions of 28°C, filling volume ml / 250m, inoculum volume 1%, and rotating speed 180r / min, yeast Y5 was fermented and cultured for comparison. Considering indicators such as bacteria content and enzymes comprehensively, weighing was carried out until 72 hours after fermentation. The culture medium most suitable for yeast Y5 fermentation was re-screened.
[0060] Optimizing fermentation medium usually adopts single factor method and orthogonal experiment design method, and single factor analysis of varia...
Embodiment 3
[0070] Embodiment 3: optimization and growth curve of yeast Y5 fermentation conditions
[0071] The optimization of the fermentation process plays a very important role in the fermentation industry, especially in the fermentation production, it is a very necessary technical means to improve the fermentation index. There are many methods of fermentation process optimization, they are not isolated, but interrelated, often a combination of multiple optimization methods, microorganisms in different stages of growth, different periods of production of target metabolites, the environmental conditions may be affected There are different requirements. Therefore, the temperature, pH value, dissolved oxygen, stirring speed, etc. should be constantly changed in the bioreactor to provide the best environmental conditions for it, and finally the maximum biomass and optimal metabolites can be obtained.
[0072] The microbial growth curve is a curve drawn with the number of microorganisms (...
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