Phase contrast microscopic imaging system and imaging method thereof

Abstract

The invention provides a phase contrast microscopic imaging system with a simple structure. The phase contrast microscopic imaging system comprises a microscope with an optical axis, a light source array, an image acquisition module and a calculation module; the microscope includes an objective lens; the light source array comprises a fixture with a through hole and two light source groups arranged around the through hole, the two light source groups emit a first light beam and a second light beam which are symmetrically arranged relative to the optical axis so as to irradiate a sample, and the light beams enter the sample, scatter, obliquely penetrate through the sample, are transmitted out and are collected by a microscope after passing through the through hole; the image acquisition module acquires a first image corresponding to the first light beam and a second image corresponding to the second light beam on the image side of the microscope; the calculation module is used for recovering the primary color component of the first image and the primary color component of the second image and subtracting the primary color component obtained by the first image from the primary colorcomponent obtained by the second image to obtain a phase contrast image. The imaging method of the phase contrast microscopic imaging system is simple and easy to operate.

Description

【Technical field】 [0001] The invention relates to the technical field of microscopic imaging, in particular to a phase contrast microscopic imaging system and an imaging method thereof. 【Background technique】 [0002] The development of microscopic imaging technology has brought a whole new world to the human eye. People can see hundreds of "new" tiny animals and plants, as well as the internal structure of everything from human bodies to plant fibers. , help scientists discover new species, and help doctors treat diseases. [0003] Phase-contrast microscopy is used to visualize unstained specimens. As the light wave passes through living cells and undyed biological specimens, its wavelength and amplitude do not change, which cannot be observed by the human eye, but its phase changes. Phase contrast microscopy changes the phase difference and uses light diffraction and interference phenomena to change the phase difference into an amplitude difference to observe living cell...

AI Technical Summary

Problems solved by technology

Existing phase-contrast microscopy techniques can only observe slices with sample thicknesses in the order of microns, and have high requirements for thickness, and cannot observe thicker sample tissues (millimeters), and have no real significance in human biopsy. application

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