Burkholderia sp. Y-4 and application thereof to adsorption of heavy metal ions and sulfamido antibiotics

A technology of heavy metal ions and Burkholderia, applied in the field of environmental pollutant treatment, can solve the problems of single function, easy loss of microbial activity, difficult separation and recovery, etc., and achieve the effect of solving pollution and significant adsorption and removal effect

Active Publication Date: 2020-03-31
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved in the present invention is to overcome the defects and deficiencies of existing microbial adsorbents such as single function, easy loss, difficult separation and recovery, and easy loss of microbial activity, and provide a strain of Burkholderia Y-4 and its ability to absorb heavy metal ions. and sulfonamide antibiotics

Method used

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  • Burkholderia sp. Y-4 and application thereof to adsorption of heavy metal ions and sulfamido antibiotics
  • Burkholderia sp. Y-4 and application thereof to adsorption of heavy metal ions and sulfamido antibiotics
  • Burkholderia sp. Y-4 and application thereof to adsorption of heavy metal ions and sulfamido antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Isolation, purification and screening of target bacterial strain Y-4

[0063] 1. Experimental method

[0064] (1) Separation and purification of bacterial strains: Collect the remaining sludge from a sewage treatment plant. First, under aseptic conditions, add the sludge sample to 100 mL of heavy metal Cd 2+ (10mg / L) nutrient medium, place 30 DEG C, 150r / min constant temperature shaker, dark culture 42h, obtain culture fluid; Culture fluid presses 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 Gradient dilution, respectively take 10 -4 、10 -5 and 10 -6 200 μL of each of the three dilution gradient culture solutions were spread and inoculated into the solid nutrient medium, and placed upside down in the incubator at 30°C for 30 hours; then, the colonies grown in the solid nutrient medium were numbered, and the colonies were picked separately. out, further streaked and cultured until a purified single colony was obtained;

[0065] (2) Strain screening: E...

Embodiment 2

[0072] The identification of embodiment 2 target strain Y-4

[0073] (1) Morphological identification of the target strain Y-4

[0074] The strain grows as pale yellow colonies on the plate, and the colonies are small, round, moist and raised; under the microscope, the cells of the strain Y-4 are small, short rod-shaped, and Gram-negative.

[0075] (2) Molecular biological identification of the target strain Y-4

[0076] 1. Experimental method

[0077] Perform 16S rRNA gene sequencing on the target strain Y-4 obtained in Example 1, entrust Shanghai Meiji Biomedical Technology Co., Ltd. to perform sequencing, sequence comparison, and construct a phylogenetic tree of the 16S rRNA gene sequence of the strain.

[0078] 2. Experimental results

[0079] The results of the phylogenetic tree constructed using the 16S rRNA gene sequence of the target strain Y-4 are as follows: image 3 As shown, it can be seen from the results of the phylogenetic tree that it is judged that the str...

Embodiment 3

[0080] The preparation of the adsorbent of embodiment 3 different concentrations Burkholderia Y-4 and its to Cd 2+ Determination of the adsorption effect of

[0081] 1. Experimental method

[0082] S1. Weigh 2.5g of sodium alginate and add it to 90mL of deionized water, heat it to a water temperature above 50°C, and stir at the same time until the sodium alginate is completely dissolved to obtain a sodium alginate solution, add 1g of powdered activated carbon, and stir to mix evenly , to obtain the mixture a;

[0083] S2. When the mixed solution a is cooled to below 45°C, add 10mL of Burkholderia Y-4 bacterial suspension with a concentration of 0, 5g / L, 10g / L, 15g / L and 20g / L respectively, and stir evenly to obtain Mixture b;

[0084] S3. Use a syringe or dropper to slowly drop the mixed solution b into 4% CaCl by mass fraction 2 solution, and then put it at 4°C for 24h to cross-link to obtain adsorbents with different concentrations of Burkholderia Y-4.

[0085] Take 150...

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Abstract

The invention discloses burkholderia sp. Y-4 and an application thereof to adsorption of heavy metal ions and sulfamido antibiotics. Through separation and screening, the burkholderia sp. (Burkholderia sp.) Y-4 is obtained, the strain is preserved in Guangdong Microbiological Culture Collection Center on 27 July 2018, and the preservation number is GDMCC NO:60423. The strain has significant adsorption and removal effects on heavy metals and sulfamido antibiotics, and can effectively control damage of the heavy metals and the sulfamido antibiotics to environment. In addition, the invention further provides a microorganism heavy metal ion adsorbent and a sulfamido antibiotic adsorbent, which have the characteristics of being convenient to feed, high in stability, significant in adsorption and removal effects, high in poison resistance capacity, low in running off of microorganisms and easy in product separation; when the adsorbents are used for treating waste water, the advantages beinglow in cost, energy-saving, environmentally-friendly, efficient, free from secondary pollution and the like can be achieved, and the burkholderia sp. Y-4 has great application prospects in removing the heavy metal ions and the sulfamido antibiotics.

Description

technical field [0001] The invention belongs to the technical field of environmental pollutant treatment. More specifically, it relates to a strain of Burkholderia Y-4 and its application in adsorbing heavy metal ions and sulfonamide antibiotics. Background technique [0002] Industrial production and various mining activities will produce wastewater containing a large amount of heavy metals, and even urban domestic sewage also contains heavy metals. Heavy metals are indispensable elements for human health, but the required amount is very small. If it exceeds the safety limit, it will cause harm to the human body and cause serious consequences; for example, the "Minamata disease" and "Bone Pain Disease" that shocked the world Water pollution caused by mercury and cadmium. Heavy metals cannot be degraded in the environment, and can be bioaccumulated through the food chain, posing a serious threat to ecological security and human health. Therefore, the treatment of heavy me...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01J20/24C02F3/34C12R1/01C02F101/20C02F101/22C02F101/30
CPCB01J20/24C02F3/34C02F2101/20C02F2101/30C02F2101/40C02F2101/22C12R2001/01C12N1/205
Inventor 陈烁娜檀笑刘慧玲韩伟江宗颖欣解启来
Owner SOUTH CHINA AGRI UNIV
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