Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of soybean mosaic virus conserved fragment CFO and reverse repeat vector constructed by same in broad-spectrum resistance of soybeans to SMV

A soybean mosaic virus, inverted repeat technology, applied in the direction of virus/phage, application, virus, etc., can solve the problems of affecting soybean yield and quality, long polymerization period, etc.

Active Publication Date: 2020-04-03
NANJING AGRICULTURAL UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the SMV resistance gene has not yet been cloned
The method of disease resistance gene aggregation can improve the disease resistance of soybean, but it will affect the yield and quality of soybean (that is, there is the cost of disease resistance) and the aggregation period is longer
In addition, SMV mutations leading to its breakthrough host resistance often occur

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of soybean mosaic virus conserved fragment CFO and reverse repeat vector constructed by same in broad-spectrum resistance of soybeans to SMV
  • Application of soybean mosaic virus conserved fragment CFO and reverse repeat vector constructed by same in broad-spectrum resistance of soybeans to SMV
  • Application of soybean mosaic virus conserved fragment CFO and reverse repeat vector constructed by same in broad-spectrum resistance of soybeans to SMV

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] CFO-TIR Vector pB7GWIWG2(II)-CFO Constructed from SMV Conserved Fragment CFO Mediates Broad Spectrum Resistance to SMV in Transgenic Soybean

[0026] (1) The conserved fragment CFO was obtained by multiple sequence alignment, identification and screening of the SMV genome:

[0027] In the NCBI database ( http: / / www.ncbi.nlm.nih.gov / ) By comparing and analyzing 30 SMV genome sequences (see Table 1 for GenBank ID).

[0028] Table 1 GenBank IDs of 30 SMV genome sequences

[0029] NC_002634.1 FJ640959.1 EU871724.1 FJ640967.1 FJ640981.1 AB100443.1 D00507.2 FJ640976.1 FJ640979.1 AJ312439.1 FJ640980.1 AY294045.1 FJ640975.1 FJ640974.1 S42280.1 FJ376388.1 FJ640957.1 FJ640971.1 FJ640972.1 FJ640966.1 FJ640955.1 FJ807701.1 FJ548849.1 FJ640954.1 FJ640968.1 EU871725.1 FJ640963.1 FJ807700.1 AY294044.1 AB100442.1

[0030] The multiple sequence alignment of these SMV genome sequences was carried out by ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of a soybean mosaic virus (SMV) conservative fragment CFO and a reverse repeat vector (CFO-TIR) constructed by the soybean mosaic virus conservative fragment CFOin broad-spectrum resistance of soybeans to SMV. According to the invention, multi-sequence alignment and conservative calculation are carried out on 30 SMV genome sequences for the first time, an SMV fragment CFO (a nucleotide sequence is shown as SEQ ID NO: 1) with the conservative property of 92% is identified, a CFO-TIR vector is constructed and genetically transformed into an SMV susceptiblesoybean variety NN1138-2, the CFO-TIR vector mediates the resistance of transgenic soybeans to multiple SMV strains, and the problem of broad-spectrum resistance of transgenic plants to SMV is solved. The transgenic material obtained by the invention has important application value for cultivating SMV broad-spectrum resistant soybeans.

Description

technical field [0001] The invention belongs to the field of soybean technology, and relates to the improvement of soybean resistance to soybean mosaic virus (SMV), in particular to a conservative fragment CFO of soybean mosaic virus (SMV) and an inverted repeat vector (CFO-TIR) constructed therefrom. Application of soybean for broad-spectrum resistance to SMV. Background technique [0002] Soybean is an important oil and protein crop in the world. During its growth cycle, it is often attacked by various diseases and insect pests. SMV is a serious viral disease that seriously affects soybean yield and quality. For the convenience of research, SMV was artificially divided into multiple strains based on the responses on different identified hosts, and then the specific resistance loci of SMV strains were identified accordingly. However, the SMV resistance gene has not yet been cloned. The method of disease resistance gene aggregation can improve the disease resistance of so...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/40C12N15/84A01H5/00A01H6/54
CPCC07K14/005C12N15/8283C12N2770/34022
Inventor 盖钧镒姜华李凯
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com