Hymenopellis raphanipes strain and breeding method thereof

A technology of A. bisporus and a strain, which is applied to the field of A. bisporus strains and their breeding, can solve problems such as less research on A. bisporus, and achieves consistent development, short growth cycle, The effect of increased biotransformation

Active Publication Date: 2020-04-10
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some strains of the genus Ouderia minor have been domesticated and cultivated domestically (Li Chuanhua et al., 2011). There are few related studies on German mushroom

Method used

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  • Hymenopellis raphanipes strain and breeding method thereof
  • Hymenopellis raphanipes strain and breeding method thereof
  • Hymenopellis raphanipes strain and breeding method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Strain isolation

[0046] Choose mushrooms without umbrellas and stalks without cracks, and discard young mushrooms eaten by ants and fruit fly larvae. After wiping the fruiting bodies of young mushrooms with sterile paper or cotton, use sterile tweezers to pick out the internal hyphae, place them in PDA medium containing streptomycin, and cultivate them at 26°C for 7 days. Colonies with high whiteness, uniform density everywhere, and no bacterial contamination around.

[0047] (2) strain purification

[0048] Pick the aerial hyphae in the target colonies from the bacterial colonies selected in step (1) and insert them into fresh PDA medium to purify the strains, and transfer the strains after 3 times of purification into inclined test tubes, Cultivate at 24°C to 26°C until the slope is covered with hyphae to obtain pure strains for strain preservation. Mycelial morphology and culture characteristics such as figure 1 shown.

[0049] figure 1 A shows that the m...

Embodiment 2

[0057] (1) Fermented cultivation material

[0058] Weigh cottonseed husks, the raw material for cultivation, add water and mix well, fully stir with a mixer during adding water to make it fully absorb water, then add an appropriate amount of lime water and mix well to make the pH value 8.0, build a heap and ferment for 8 days, remove The gossypol in the cottonseed shell increases the water holding capacity of the material and softens the material.

[0059] Preparation of solid medium: 85.2% of fermented cottonseed hulls, 10.2% of wheat bran, 2.6% of glucose, 0.3% of magnesium sulfate, 1.2% of potassium dihydrogen phosphate, and 0.5% of calcium carbonate; the water content is 60% to 75%, and the pH is natural Weighing the solid culture medium according to the ratio of parts by mass, adding water and mixing well, fully stirring with a mixer during adding water to make it fully absorb water, adjusting the water content of the solid culture medium to 68%, and pH 8.5.

[0060] (2)...

Embodiment 3

[0092] The pure mycelia obtained in the step (2) of Example 1 was inoculated onto the medium I (malt extract 5g / L, maltose 10g / L, yeast extract 2.5g / L, ammonium sulfate 0.5g / L, sulfuric acid Magnesium 1.7g / L, potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, agar 20g / L, distilled water to 1000mL, pH natural), at 20°C, 22°C, 24°C, 26°C , 28°C and 30°C for 7 days at a constant temperature and protected from light, measure the diameter of the colony, and calculate the daily average growth rate. The result is as Figure 7 As shown, it can be seen that Oddya bisporus can grow in the temperature range of 20°C to 30°C, and the growth rate is the fastest at 26°C, and the optimum growth temperature range is 24°C to 26°C, which is lower than the temperature range of 26°C. The hyphae of O. bisporus grow slowly at 22℃ and higher than 28℃.

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Abstract

The invention discloses a hymenopellis raphanipes strain and a breeding method thereof. The strain provided by the invention is named hymenopellis raphanipes MesAdm 1, the preservation number is GDMCCNo:60886, and the strain is preserved at Guangdong Microbial Culture Collection Center in building 59, No.100, Xianlie Middle Road, Guangzhou on October 29, 2019. The growth rate of the hymenopellisraphanipes strain provided by the invention is fast and can reach 8.2000 +/- 0.2830 mm/d, the strain can produce mushrooms without covering soil, the yield is up to 17-22 mushrooms/bottle, and the growth period is short and can reach 58 days; and fruit body of the hymenopellis raphanipes strain provided by the invention is umbrella-shaped, the length of each stipe is 15-23 cm, the diameter of eachstipe is 0.5-1.5 cm, the diameter of each pileus is 4.0-12 cm, the pilei are uniform and moderate, the quality is good, the quality is stable, and the development is consistent.

Description

technical field [0001] The invention relates to the technical field of edible fungus breeding, in particular to a Pseudomonas bisporus strain and a breeding method thereof. Background technique [0002] Hymenopellis raphanipes belongs to Basidiomycota, Agaricales, pHysalacriceae, Oudemansiella. In recent years, some strains of the genus Ouderia minor have been domesticated and cultivated domestically (Li Chuanhua et al., 2011). There are few related studies on the mushroom. Contents of the invention [0003] The primary purpose of the present invention is to provide a strain of Pseudomonas bisporus. [0004] Another object of the present invention is to provide a breeding method for the above-mentioned Pseudomonas bisporus strain. [0005] The object of the present invention is achieved through the following technical solutions: a strain of Pseudomonas bisporus, called Hymenopellis raphanipes MesAdm 1, and the preservation number is GDMCC No: 60886, which was released o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02A01H15/00C12R1/645
CPCA01H15/00C12N1/02C12N1/14C12N1/145C12R2001/645
Inventor 莫美华王越袁征超苏楚亮杨昱仪卢意薛玲娜查丽燕张振伟文华枢侯跃辉宋舒晴
Owner SOUTH CHINA AGRI UNIV
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