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Agaricus blazei murill small peptide, and preparation method and application thereof

A technology of Agaricus blazei and peptides, applied in the field of natural active products, to reduce cell damage, improve learning ability, and improve animal memory impairment

Active Publication Date: 2020-04-14
BEIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no small molecular peptide derived from Agaricus blazei that has a good application prospect.

Method used

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  • Agaricus blazei murill small peptide, and preparation method and application thereof
  • Agaricus blazei murill small peptide, and preparation method and application thereof
  • Agaricus blazei murill small peptide, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Preparation of agaricus blazei small peptide.

[0042] 1. Extraction.

[0043] The powdery fruiting bodies of Agaricus blazei (about 3000g) were homogenized on ice in 5000mL distilled water, centrifuged at 5000r / min for 15min to get the supernatant, and then ammonium sulfate ((NH 4 )2SO 4 To a concentration of 75-85 wt%, the soluble protein in the supernatant was precipitated overnight at 4°C, and the obtained precipitate A was centrifuged.

[0044] 2. Dialysis.

[0045] The above precipitate was taken, dissolved in deionized water, and dialyzed with deionized water at 4°C for 24 hours using a dialysis membrane with a molecular weight cut-off of 30kDa, and then freeze-dried to obtain a dialyzed protein powder.

[0046] 3. Enzyme hydrolysis.

[0047] Take the above-mentioned dialysis protein powder, add distilled water at 1:20 (g / mL), adjust the pH value to 2.5 with 0.1mol / L hydrochloric acid (HCl), add trypsin hydrolyzate according to the enzyme amount of 3000u / mg,...

Embodiment 2

[0079] active screening.

[0080] 1. Method.

[0081] Take each component in the process of preparing the Agaricus blazei small peptide, see the table below for details, and measure its activity according to the following method.

[0082] Determination of DPPH free radical scavenging ability:

[0083] (1) Dilute Agaricus blazei polypeptide solution to an appropriate concentration.

[0084] (2) Weigh 7.88mg of DPPH, dilute it with absolute ethanol in a 100mL volumetric flask, shake well and set aside.

[0085] (3) Take 100 μL samples and add them to 96-well plates, then add DPPH 100 μL, make 3 parallel samples in the sample group, control group (Axo), and blank group (Ao), shake well, wrap in tin foil and carry out Protect from light, and place in a 25°C incubator for 30 minutes. Absorbance values ​​(Ax) were measured at 517 nm. The formula for calculating the DPPH clearance rate is:

[0086]

[0087] 2. Results.

[0088] The activity results are shown in the table be...

Embodiment 3

[0094] Research on the protective effect and mechanism of agaricus blazei small peptide on improving D-gal-induced aging mice.

[0095] 1. Method.

[0096] 1. Grouping and administration of experimental animals

[0097] 52 male ICR mice aged 6w were selected, fed for 7 days, and randomly divided into 4 groups, 13 mice in each group: blank control group (Con), model group (Mod), Agaricus blazei small peptide group (ABp) and positive drug (Pyridine Piracetam (Pir)) group. Except for the Con group, the mice in the other groups were subcutaneously injected with D-gal 300 mg / kg at a fixed time every day, the mice in the Con group were injected with the same dose of normal saline, and the mice in the Pir group were also intragastrically administered 800 mg / kg of piracetam, ABp Group 400mg / kg agaricus blazei small peptide was administered orally every day. The Con group and the Mod group were given the same amount of distilled water by intragastric administration every day for 42 ...

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Abstract

The invention relates to an Agaricus blazei murill small peptide, and a preparation method and an application thereof, and belongs to the technical field of natural active products. The Agaricus blazei murill small peptide comprises a peptide fragment with the amino acid sequence reprensted by SEQ ID No. 1. The Agaricus blazei murill small peptide is obtained through enzymolysis and chromatographic column purification, and the main peptide fragment sequence of the Agaricus blazei murill small peptide is RQR (arginine-glutamine-arginine); and animal experiments prove that the Agaricus blazei murill small peptide can improve the learning and memory ability of mice in a Morris water maze, detection of biochemical indexes in serum of mice shows that the Agaricus blazei murill small peptide cansignificantly improve the oxidative stress level in the mice, the CAT and T-AOC values are significantly increased, and the ROS and MDA contents are reduced, that is, the Agaricus blazei murill smallpeptide can improve animal dysmnesia and improve learning ability, and has an obvious anti-aging effect. Experiments show that the Agaricus blazei murill small peptide has good effects of improving dysmnesia, improving learning ability and resisting aging, has the advantages of short peptide fragment, good solubility and good absorption, and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of natural active products, in particular to a small peptide of Agaricus blazei and its preparation method and application. Background technique [0002] The aging of human cells and tissues refers to the degenerative changes in structure and function. During the process, the nervous system gradually declines, resulting in behavioral disorders, learning and memory decline, and other phenomena. The increased degree of oxidative stress in the body is an important cause of aging, and the degree of oxidative stress depends on the balance of oxidation and antioxidant systems. Therefore, improving the body's antioxidant capacity can delay the aging process. Natural products contain a large number of antioxidant active substances, and their use to prevent aging and related diseases has become a hot spot in the field of modern biological research. [0003] As an important source of natural products, fungi have sho...

Claims

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Application Information

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IPC IPC(8): C07K5/09C07K1/36C07K1/30C07K1/18C07K1/16C12P21/06A61K38/06A61P25/00A61P39/06
CPCA61K38/00A61P25/00A61P39/06C07K5/0817C12P21/06
Inventor 安丽萍杜培革卢学春冯晴霞张倩苑广信盛瑜郭笑韩笑徐广宇孙晶波王曼力赵南晰
Owner BEIHUA UNIV
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