A kind of genetic engineering bacterium producing L-cysteine, construction method and application

A technology of genetically engineered bacteria and cysteine ​​is applied in the application field of genetically engineered bacteria in the production of L-cysteine ​​by microbial fermentation, which can solve the problem of the lack of in-depth and meticulous research on the production of L-cysteine ​​by fermentation, etc. question
CN111019877BActive Publication Date: 2022-04-19ZHEJIANG UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV OF TECH
Publication Date
2022-04-19

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Abstract

The invention discloses a genetic engineering bacterium of L-cysteine ​​and a construction method thereof, and an application of the genetic engineering bacterium in preparation of L-cysteine ​​by microbial fermentation. The present invention strengthens the serine module of Escherichia coli L-cysteine ​​synthesis pathway by (1) enhancing the ability of Escherichia coli to utilize serine; Influence, (3) increase the expression of sulfur module synthesis gene (4) heterologously express the serC gene of Corynebacterium glutamicum on the plasmid, obtain the Escherichia coli genetically engineered strain of L-cysteine ​​high production, L-cysteine ​​output from 2.7 g / L increased to 3.83g / L.
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Description

(1) Technical field

[0001] The invention relates to a genetically engineered bacterium producing L-cysteine ​​and a construction method thereof, as well as the application of the genetically engineered bacterium in preparing L-cysteine ​​by microbial fermentation. (2) Background technology

[0002] As an important sulfur-containing amino acid, L-cysteine ​​plays an important physiological role in organisms; it is not only a precursor substance for the synthesis of certain amino acids; Folding, assembly, stability, etc. play an important role. The use of L-cysteine ​​is mainly concentrated in 4 industries: food industry - used as a bread improver; cosmetics industry - used for beauty water, perm liquid, skin care cream for sun protection; pharmaceutical industry - Used as a expectorant to treat bronchitis; feed industry - used as a feed additive. According to statistics, the annual demand for L-cysteine ​​is about 5,000 tons, and the demand continues to rise.

[0003] At p...

Claims

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