Breeding method of rice variety high in resistant starch and low in gluten polymerization
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A technology of highly resistant starch and resistant starch, which can be applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial determination/inspection, etc., can solve problems such as increasing the burden on kidneys, and achieve the effect of important application value
Active Publication Date: 2020-05-01
SHANGHAI ACAD OF AGRI SCI
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However, although rice with higher gluten content has higher nutritional value, for patients with kidney disease or diabetic patients with renal dysfunctio
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Embodiment 1
[0078] Example 1 Breeding method - preferential selection of low gluten genotype homozygous
[0079] (1) In the winter of 2016, Hainan, using Youtangdao No. 2 (about 13% of resistant starch content; about 5.6% of glutenin content) , the resistant starch content is about 0.46%) is the parent II, and the F1 generation seeds are obtained by crossing.
[0080] (2) In the spring of 2017, in Shanghai, the F1 generation was backcrossed with the parent II to obtain BC1F1 generation seeds.
[0081] (3) In the winter of 2017, Hainan, sowed BC1F1 in the field, took samples, extracted DNA, and screened for resistant starch sbe3-rs genotype heterozygosity and low gluten through the detection of high resistant starch CAPS molecular marker and low gluten protein KASP molecular marker Individual plants homozygous for the Lgc-1 genotype were mixed and harvested to obtain BC1F2 seeds.
[0082] (4) In the spring of 2018, BC1F2 generation was planted in Shanghai, samples were taken, DNA was ex...
Embodiment 2
[0084] Example 2 Breeding method of the present invention - preferential selection of resistant starch genotype homozygous
[0085] (1) In the winter season of 2016, Hainan, using Youtangdao No. 2 (same as Example 1) as parent I, and Japanese low gluten variety LGC-1 (same as Example 1) as parent II, hybridized to obtain F1 generation seeds (same as Example 1) Embodiment 1 step 1).
[0086] (2) In the winter and spring of 2017, in Shanghai, the F1 generation was backcrossed with the parent I to obtain BC1F1 generation seeds. The BC1F1 generation seeds were ground into brown rice, and the chalkiness of grains greater than 70% was selected according to the appearance, planted in Hainan, sampled, and DNA extracted. Through the detection of high resistant starch CAPS molecular markers and low gluten protein KASP molecular markers, single plants homozygous for sbe3-rs genotype and heterozygous Lgc-1 genotype were screened, and BC1F2 generation seeds were obtained by mixing and ha...
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Abstract
The invention discloses a breeding method of a rice variety high in resistant starch and low in gluten polymerization. The breeding method comprises the steps of using a rice variety high in resistantstarch content as parents I, using a variety low in gluten content as parents II, and performing hybridization to obtain F1-generation seeds; performing backcross on the F1-generation seeds and the parents I to obtain BC1F1-generation seeds, and selecting BC1F1 seeds of which the chalkiness is higher than 70%; after sowing, detecting the BC1F1-generation seeds with a resistant starch molecular marker and a low-gluten molecular marker, performing screening to obtain single plants of which high resistant starch genotypes are in homozygosis and low gluten genotypes are in heterozygosis, and performing selfing to obtain BC1F2-generation seeds; and planting the BC1F2-generation seeds, performing detection through the low gluten molecular marker, performing screening to obtain strains of whichthe resistant starch genotypes and low gluten genotypes are in homozygosis, performing detection on the obtained seeds to screen strains of which the resistant starch content is larger than 10% and gluten content is lower than 3%, performing continuous selfing on the BC1F3-generation strains obtained through screening to BC1F5-generation strains, so as to obtain new rice varieties high in resistant starch and low in gluten, which are high in resistant starch content and low in gluten content and take account of yield. The rice variety bred by the breeding method disclosed by the invention hasthe characteristics of being high in resistant starch content and low in gluten content, and are suitable for diabetics and nephrotic patients needing to control protein intake to eat.
Description
technical field [0001] The invention relates to the technical field of rice breeding, in particular to a rice variety polymerized with high resistant starch and low gluten protein suitable for diabetics and kidney disease patients and a breeding method thereof. Background technique [0002] The latest data show that the number of diabetic patients in China has reached 114 million, accounting for about one-third of the total number of diabetic patients in the world. my country has become the country with the largest number of diabetic patients in the world. What is even more worrying is that the awareness rate of diabetes in my country is only about 40%. In addition to the various inconveniences that diabetes itself brings to the lives of patients, what is even more frightening is that diabetes can also bring about various complications, such as cataracts, kidney disease, diabetic foot, peripheral neuropathy, etc. [0003] Resistant starch has important physiological functio...
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