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Bonded selenium polysaccharide and its preparation method and application

A selenium polysaccharide and bonding technology, which is applied in the field of biomedicine, can solve the problems such as the difficulty of completely removing toxic inorganic selenium components, the lack of reliable evaluation of biological safety, and the normal metabolic balance of difficult selenium content, so as to improve bioavailability and avoid The risk of DNA methylation and the effect of improving immunity

Active Publication Date: 2020-08-25
ZHENGZHOU YUHEYUAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is the use of inorganic sodium selenite, but due to the lack of reliable evaluation of its biological safety, the use of inorganic sodium selenite has food safety hazards
The second is that the selenium-rich crops planted in selenium-rich soil have good product safety, but due to the limited physiological needs of the crops themselves, the selenium content in the crops is low, and it is difficult to quickly achieve the normal metabolic balance of the necessary selenium content in the blood
The third is to prepare selenium-enriched products by chemical methods, mainly through the random esterification reaction of sodium selenite and concentrated nitric acid on polysaccharide hydroxyl groups. Some relatively large amounts of toxic inorganic selenium components are difficult to completely remove

Method used

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  • Bonded selenium polysaccharide and its preparation method and application
  • Bonded selenium polysaccharide and its preparation method and application
  • Bonded selenium polysaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Synthesis of k-carrageenan-bonded selenium polysaccharide

[0045] (1) 100g of k-carrageenan polysaccharide is suspended in 200ml of dimethylformamide (DMF), and 50ml of triethylamine (Et 3N), under the condition of maintaining 0 ℃, dropwise add 25ml of chloroacetyl chloride, add Et during the reaction process 3 The amount of N should keep the pH value of the solution in a slightly alkaline state. After 4 hours the reaction was complete, the product was dissolved in water and the product was precipitated in cold ethanol.

[0046] (2) above-mentioned crude product is re-dissolved in 50ml DMF, adds the catalyst ruthenium dioxide (RuO2) of 5% weight ratio 2 ), then add 30ml concentration of 5% sodium hypochlorite (NaClO) aqueous solution under the condition of maintaining 0°C, react for 5 hours and filter the product first, and then precipitate the intermediate in ethanol at low temperature.

[0047] (3) The above reaction intermediate was dissolved in 50ml of DMF, and ...

Embodiment 2

[0052] Synthesis of loca-carrageenan-bonded selenium polysaccharide

[0053] (1) 100g of l-carrageenan polysaccharide was suspended in 200ml of dimethylformamide (DMF), and 50ml of triethylamine (Et 3 N), under the condition of maintaining zero degree, dropwise add chloroacetyl chloride 20ml, add Et in the reaction process 3 The amount of N should keep the pH value of the solution in a slightly alkaline state. After 4 hours the reaction was complete and the product was dissolved in water and the product was precipitated in cold ethanol.

[0054] (2) above-mentioned crude product is re-dissolved in 50ml DMF, adds the catalyst ruthenium dioxide (RuO2) of 7% weight ratio 2 ), then add 40ml concentration of 3% sodium hypochlorite (NaClO) aqueous solution under the condition of maintaining 0°C, react for 5 hours and filter the product first, and then precipitate the intermediate in ethanol at low temperature.

[0055] (3) The above reaction intermediate was dissolved in 50ml of ...

Embodiment 3

[0060] Synthesis of Theta-carrageenan-bonded selenium polysaccharide

[0061] (1) 200g of t-carrageenan polysaccharide is suspended in 200ml of dimethylformamide (DMF), and 100ml of triethylamine (Et 3 N), under the condition of maintaining zero degree, dropwise add chloroacetyl chloride 40ml, add Et in the reaction process 3 The amount of N should keep the pH value of the solution in a slightly alkaline state. After 5 hours the reaction was complete and the product was dissolved in water and the product was precipitated in cold ethanol.

[0062] (2) above-mentioned crude product is re-dissolved in 80ml DMF, adds the catalyst ruthenium dioxide (RuO2) of 4% weight ratio 2 ), then add 50ml concentration of 4% sodium hypochlorite (NaClO) aqueous solution under the condition of maintaining 0°C, react for 5 hours and filter the product first, and then precipitate the intermediate in ethanol at low temperature.

[0063] (3) The above reaction intermediate was dissolved in 80ml of...

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Abstract

The present invention belongs to the field of biopharmaceuticals, specifically relating to a bound selenium polysaccharide. The polysaccharide uses as a carrier a polysaccharide substance having a 3,6-levoglucosan structure, and a selenium atom connects to a carbon atom at position 6 of a polysaccharide 3,6-inner ether structure by means of a selenoester structure. By means of oxidizing the carbon atom at position 6 of the polysaccharide 3,6-inner ether bond, converting same to a 3,6-lactone, then undergoing ring-opening esterification by reacting with selenocysteine, the prepared structure is well-defined, being a bound selenium polysaccharide product containing a controllable amount of selenium. The prepared bound selenium product is able to noticeably improve the immunity of test subjects by means of increasing blood-selenium content.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a novel bonded selenium polysaccharide, its preparation method, and its application in immunity. Background technique [0002] Selenium is a trace element that is very important to the human body. Selenium cysteine ​​is the 21st amino acid. Selenium is an important part of the 25 known selenoprotein structures. Its activity depends on the existence of selenium. For example, the research The authors found that selenium is an essential component of glutathione peroxidase in animals. However, there are very limited ways for the human body to supplement selenium, and the commonly used so-called food supplements are far from satisfying the normal selenium metabolism level of the human body. For this reason, researchers first clearly pointed out that selenium is an essential trace element for the human body in 1975, and the Chinese Nutrition Society also listed selenium as one o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04A61P39/06
CPCA61K31/715A61P37/04A61P39/06C08B37/0042
Inventor 程青格董淑敏
Owner ZHENGZHOU YUHEYUAN BIOTECHNOLOGY CO LTD
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